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TB5

Name: TB5
Brand: TargetMol
SKU: T3596
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3596Cas No. 948841-07-4

TB5 is a potent, selective, and reversible inhibitor of hMAO-B.

TB5

😃Good

Purity: 99.61%

Catalog No. T3596Cas No. 948841-07-4

TB5 is a potent, selective, and reversible inhibitor of hMAO-B.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$41	In Stock	In Stock
10 mg	$66	In Stock	In Stock
25 mg	$121	In Stock	In Stock
50 mg	$197	In Stock	In Stock
100 mg	$316	-	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.61%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	TB5 is a potent, selective, and reversible inhibitor of hMAO-B.
Targets&IC50	MAO-B (human):0.11±0.01 μM. (Ki)
In vitro	TB5 and TB8 interact competitively with the catalytic sites of hMAO-B and hMAO-A. TB5 shows the highest inhibitory activity and selectivity toward hMAO-B, with Ki and SI values of 0.11±0.01 μM and 13.18, respectively. Inhibition by TB5 (hMAO-B) and TB8 (hMAO-A) is fully reversible after 24 hours of dialysis. Cytotoxicity studies reveal TB5 is non-toxic at 5 and 25 μM, with cell viabilities of 95.75% and 84.59%, respectively [1].
In vivo	Compounds are initially dissolved in DMSO (5 mg/mL), then further diluted with a PBS/EtOH mixture (70:30). To elucidate the critical interactions dictating selectivity and potency, kinetic analyses of TB5 and TB8 are conducted. Lineweaver–Burk plots, a total of five, are created to compare the catalytic rates of MAO-B and MAO-A at various substrate concentrations (0.1-1 μM) in both the absence and presence of TB5 and TB8 at differing concentrations. The initial plot reveals the baseline enzyme activity without inhibitors, while the subsequent four plots show the impact of varying concentrations of TB5 and TB8[1].
Kinase Assay	Compounds are dissolved in DMSO (5 mg/mL) and diluted with PBS/EtOH (70:30).Kinetic analyses are carried out for TB5 and TB8. A set of Lineweaver–Burk plots are constructed in the absence and presence of various concentrations of compounds TB5 and TC8. The set consists of five graphs, each constructed by measuring MAO-B and MAO-A catalytic rates at different substrate concentrations (0.1-1 μM). The first Lineweaver–Burk plot is constructed in the absence of inhibitor, while the remaining four graphs are constructed in the presence of different concentrations of TB5 and TB8[1].
Cell Research	In vitro cytotoxicity of brominated thiophene chalcones and standard MAO inhibitors are tested in human HepG2 hepatic cancer cells at three different concentrations (1, 5 and 25 μM)[1].

Chemical Properties

Molecular Weight	336.25
Formula	C₁₅H₁₄BrNOS
Cas No.	948841-07-4
Smiles	CN(C)c1ccc(\C=C\C(=O)c2ccc(Br)s2)cc1
Relative Density.	1.440 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (163.57 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9740 mL	14.8699 mL	29.7398 mL	148.6989 mL
5 mM	0.5948 mL	2.9740 mL	5.9480 mL	29.7398 mL
10 mM	0.2974 mL	1.4870 mL	2.9740 mL	14.8699 mL
20 mM	0.1487 mL	0.7435 mL	1.4870 mL	7.4349 mL
50 mM	0.0595 mL	0.2974 mL	0.5948 mL	2.9740 mL
100 mM	0.0297 mL	0.1487 mL	0.2974 mL	1.4870 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc