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SCH900776

Name: SCH900776
Brand: TargetMol
SKU: T2517
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2517Cas No. 891494-63-6

Alias MK-8776, MK 8776

SCH900776 (MK-8776) is an agent targeting cell cycle checkpoint kinase 1 (Chk1) with potential radiosensitization and chemosensitization activities.

SCH900776

🥰Excellent

Purity: 99.6%

Catalog No. T2517Alias MK-8776, MK 8776Cas No. 891494-63-6

SCH900776 (MK-8776) is an agent targeting cell cycle checkpoint kinase 1 (Chk1) with potential radiosensitization and chemosensitization activities.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$35	In Stock	In Stock
5 mg	$78	In Stock	In Stock
10 mg	$152	In Stock	In Stock
25 mg	$297	In Stock	In Stock
50 mg	$486	In Stock	In Stock
100 mg	$695	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$85	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.6%

Color:White

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Product Introduction

Bioactivity

Description	SCH900776 (MK-8776) is an agent targeting cell cycle checkpoint kinase 1 (Chk1) with potential radiosensitization and chemosensitization activities.
Targets&IC50	Chk1:3 nM, CDK2:0.16 μM, Chk2:1500 nM
In vitro	Administering 4 mg/kg of SCH 900776 30 minutes after gemcitabine triggers the γ-H2AX biomarker, while a dose of 8 mg/kg of SCH 900776 boosts tumor pharmacodynamics and degradation response. Incremental increases to 16 mg/kg and 32 mg/kg of SCH 900776 further improve tumor response. Notably, within BALB/c mice, the dose of SCH 900776 correlates with strong biomarker activation, independent of gemcitabine's intensified hematologic toxicity.
In vivo	After 24 hours of exposure to hydroxyurea, SCH 900776 induces a dose-dependent loss of DNA replication capacity. SCH 900776 enhances the response of γ-H2AX to hydroxyurea, 5-fluorouracil, and cytarabine. By combining with antimetabolites, SCH 900776 triggers the accumulation of γ-H2AX within 2 hours, indicating replication fork collapse and subsequent double-strand DNA breaks. Additionally, SCH 900776 inhibits the accumulation of Chk1 pS296 autophosphorylation in a dose-dependent manner. SCH 900776 is a weak inhibitor of Chk2 and CDK2, with IC50 values of 1.5 μM and 0.16 μM, respectively. SCH 900776 does not significantly inhibit human liver microsomal cytochrome P450 subtypes 1A2, 2C9, 2C19, 2D6, and 3A4.
Kinase Assay	The Millipore Kinase Profiler service is used to generate general selectivity data for SCH 900776 against a broad range of serine/threonine and tyrosine kinases. Assays are typically run at two concentrations of SCH 900776 (0.5 and 5 μM), at a fixed (10 μM) concentration of ATP. Data are provided as percent activity remaining, relative to uninhibited controls.
Cell Research	For cell growth assays, cells are seeded at low density (500-1000 cells) in 96-well plates and then incubated with drug for 24 h (8 wells per concentration). Following treatment, cells are washed and grown in fresh media for 5-7 days at 37°C. Prior to attaining confluence, cells are washed, lysed, and stained with Hoechst 33258. Fluorescence is read on a microplate spectrofluorometer. Results are expressed as mean and standard error for the concentration of drug that inhibited growth by 50%.
Synonyms	MK-8776, MK 8776

Chemical Properties

Molecular Weight	376.25
Formula	C₁₅H₁₈BrN₇
Cas No.	891494-63-6
Smiles	Cn1cc(cn1)-c1cnn2c(N)c(Br)c(nc12)[C@@H]1CCCNC1
Relative Density.	1.80 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (265.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (10.63 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6578 mL	13.2890 mL	26.5781 mL	132.8904 mL
5 mM	0.5316 mL	2.6578 mL	5.3156 mL	26.5781 mL
10 mM	0.2658 mL	1.3289 mL	2.6578 mL	13.2890 mL
20 mM	0.1329 mL	0.6645 mL	1.3289 mL	6.6445 mL
50 mM	0.0532 mL	0.2658 mL	0.5316 mL	2.6578 mL
100 mM	0.0266 mL	0.1329 mL	0.2658 mL	1.3289 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc