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Abemaciclib methanesulfonate

Name: Abemaciclib methanesulfonate
Brand: TargetMol
SKU: T3111
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T3111Cas No. 1231930-82-7

Alias LY2835219 mesylate, LY2835219 (methanesulfonate), LY2835219, abemaciclib mesylate

Abemaciclib methanesulfonate (LY2835219) is a specific and effective inhibitor of CDK4(IC50=2 nM) and CDK6(IC50=10 nM).

Abemaciclib methanesulfonate

🥰Excellent

Purity: 99.44%

Catalog No. T3111Alias LY2835219 mesylate, LY2835219 (methanesulfonate), LY2835219, abemaciclib mesylateCas No. 1231930-82-7

Abemaciclib methanesulfonate (LY2835219) is a specific and effective inhibitor of CDK4(IC50=2 nM) and CDK6(IC50=10 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$50	In Stock	In Stock
10 mg	$72	In Stock	In Stock
25 mg	$86	In Stock	In Stock
50 mg	$98	In Stock	In Stock
100 mg	$126	In Stock	In Stock
200 mg	$157	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$67	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.44%

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Abemaciclib methanesulfonate (LY2835219) is a specific and effective inhibitor of CDK4(IC50=2 nM) and CDK6(IC50=10 nM).
Targets&IC50	CDK6:10 nM, GSK-3β:192 nM, PIM1:50 nM, PIM2:3400 nM, HIPK2:31 nM, CDK1-cyclinB1:1627 nM, CK2:117 nM, FLT3 (D835Y):403 nM, JNK3:389 nM, CDK2-CyclinE:504 nM, CDK9-CyclinT1:57 nM, DRAK1:659 nM, CDK5-p35:287 nM, CDK7-CyclinH-MAT1:3910 nM, CDK4:2 nM, DYRK2:61 nM, FLT3:3960 nM, CDK5-p25:355 nM
In vitro	In subcutaneous and intracranial glioma models (U87 mg), MsOH treatment results in LY2835219 inhibiting tumor growth in a dose-dependent manner.
In vivo	In retinoblastoma, LY2835219 inhibits cancer cell growth by specifically targeting and inhibiting CDK4 and CDK6, leading to cell cycle arrest at the G1 phase and suppression of DNA synthesis.
Kinase Assay	Cells (5 × 103) are plated in 96 well plates. Cells are treated the next day for 24 to 48 hours and then assessed for caspase-3 activity by Caspase-Glo-3/7 Assay, as per manufacturer's instructions and a luminescence plate reader.
Cell Research	LY2835219 is dissolved in DMSO to a 10 mM concentration.? Cells are seeded in a 96-well plate, allowed to adhere overnight, and treated with DMSO control (0.1% v/v) or the indicated compounds for 72 h. Cell viability and proliferation are determined using a Cell Counting Kit according to the manufacturer's instructions. The interaction between LY2835219 and mTOR inhibitor is determined using CompuSyn. Combination index (CI) values of 1 indicates and additive drug interaction, whereas a CI of < 1 is synergistic and a CI of > 1 is antagonistic.
Synonyms	LY2835219 mesylate, LY2835219 (methanesulfonate), LY2835219, abemaciclib mesylate

Chemical Properties

Molecular Weight	602.7
Formula	C₂₇H₃₂F₂N₈·CH₄O₃S
Cas No.	1231930-82-7
Smiles	CS(O)(=O)=O.CCN1CCN(Cc2ccc(Nc3ncc(F)c(n3)-c3cc(F)c4nc(C)n(C(C)C)c4c3)nc2)CC1
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 45 mg/mL (74.66 mM), Sonication is recommended.

DMSO: 9.62 mg/mL (15.96 mM), Sonication is recommended.

Ethanol: 23 mg/mL (38.16 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (1.66 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol/H2O

	1mg	5mg	10mg	50mg
1 mM	1.6592 mL	8.2960 mL	16.5920 mL	82.9600 mL
5 mM	0.3318 mL	1.6592 mL	3.3184 mL	16.5920 mL
10 mM	0.1659 mL	0.8296 mL	1.6592 mL	8.2960 mL

Ethanol/H2O

	1mg	5mg	10mg	50mg
20 mM	0.0830 mL	0.4148 mL	0.8296 mL	4.1480 mL

H2O

	1mg	5mg	10mg	50mg
50 mM	0.0332 mL	0.1659 mL	0.3318 mL	1.6592 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc