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Anti-CDK2 Polyclonal Antibody 2
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Catalog No. TMAB-00404
Anti-CDK2 Polyclonal Antibody 2 is a Rabbit antibody targeting CDK2. Anti-CDK2 Polyclonal Antibody 2 can be used in WB,FCM.
Anti-CDK2 Polyclonal Antibody 2
Anti-CDK2 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00404
Anti-CDK2 Polyclonal Antibody 2 is a Rabbit antibody targeting CDK2. Anti-CDK2 Polyclonal Antibody 2 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Blank control (Black line): U87Mg (Black). Primary Antibody (green line): Rabbit Anti-CDK2 antibody (TMAB-00404) Dilution: 3 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1: LympHnode (Mouse) Lysate at 40 μg Lane 2: Esophagus (Mouse) Lysate at 40 μg Lane 3: K562 (Human) Cell Lysate at 30 μg Lane 4: Molt-4 (Human) Cell Lysate at 30 μg Lane 5: Siha (Human) Cell Lysate at 30 μg Primary: Anti-CDK2 (TMAB-00404) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 3μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human CDK2 |
| Antigen Species | Human |
| Gene ID | 1017 |
| Uniprot ID | |
| Biology Area | Cdks,Cdks,Cdks |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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