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PIM447

Name: PIM447
Brand: TargetMol
SKU: T12475
Price: 113 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T12475Cas No. 1210608-43-7

Alias PIM 447, LGH447, LGH 447

PIM447 (LGH447) is an oral, selective pan-PIM kinase inhibitor with antitumor and bone-protective effects. It inhibits PIM1, PIM2, and PIM3, induces apoptosis, and decreases the viability, proliferation, and motility of HuH6 and COA67 cells. PIM447 suppresses tumorigenesis in hepatocellular carcinoma and can be used in the study of multiple myeloma.

PIM447

🥰Excellent

Purity: 98.97%

Catalog No. T12475Alias PIM 447, LGH447, LGH 447Cas No. 1210608-43-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$113	In Stock	In Stock
5 mg	$278	In Stock	In Stock
10 mg	$455	In Stock	In Stock
25 mg	$767	In Stock	In Stock
50 mg	$987	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$297	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.97%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	PIM447 (LGH447) is an oral, selective pan-PIM kinase inhibitor with antitumor and bone-protective effects. It inhibits PIM1, PIM2, and PIM3, induces apoptosis, and decreases the viability, proliferation, and motility of HuH6 and COA67 cells. PIM447 suppresses tumorigenesis in hepatocellular carcinoma and can be used in the study of multiple myeloma.
Targets&IC50	Pim2:18 pM (Ki), Pim1:6 pM (Ki), Pim3:9 pM (Ki)
In vitro	HuH6 and COA67 cells were treated with PIM447 (0-20 μM, 72h), and the activity of PIM447 was significantly reduced with LD50 of 13 μM and 10 μM, respectively. Treatment with PIM447 (7.5 μM, 8h) resulted in increased apoptosis of HuH6 and COA67 cells. PIM447 (5 μM, 48h) reduced cancer cell dryness in HuH6 and COA67 cells. [1]
In vivo	PIM447 significantly reduced tumor load and prevented tumor-associated bone loss in a disseminated mouse model of human myeloma. [2]
Synonyms	PIM 447, LGH447, LGH 447

Chemical Properties

Molecular Weight	440.46
Formula	C₂₄H₂₃F₃N₄O
Cas No.	1210608-43-7
Smiles	FC1=C(N=C(C(NC=2C(=CC=NC2)[C@@H]3C[C@H](C)C[C@H](N)C3)=O)C=C1)C4=C(F)C=CC=C4F
Relative Density.	1.290 g/cm3 (Predicted)
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (181.63 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (11.35 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2704 mL	11.3518 mL	22.7035 mL	113.5177 mL
5 mM	0.4541 mL	2.2704 mL	4.5407 mL	22.7035 mL
10 mM	0.2270 mL	1.1352 mL	2.2704 mL	11.3518 mL
20 mM	0.1135 mL	0.5676 mL	1.1352 mL	5.6759 mL
50 mM	0.0454 mL	0.2270 mL	0.4541 mL	2.2704 mL
100 mM	0.0227 mL	0.1135 mL	0.2270 mL	1.1352 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc