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Osalmid

Name: Osalmid
Brand: TargetMol
SKU: T0353
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0353Cas No. 526-18-1

Alias Oxaphenamide, 4'-Hydroxysalicylanilide

Osalmid (Oxaphenamide) is a choleretic drug, inhibits ribonucleotide reductase activity by targeting ribonucleotide reductase small subunit M2 (RRM2).

Osalmid

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Purity: 99.6%

Catalog No. T0353Alias Oxaphenamide, 4'-HydroxysalicylanilideCas No. 526-18-1

Osalmid (Oxaphenamide) is a choleretic drug, inhibits ribonucleotide reductase activity by targeting ribonucleotide reductase small subunit M2 (RRM2).

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$50	In Stock	In Stock
1 g	$85	In Stock	In Stock
2 g	$162	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.6%

Appearance:Solid

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Osalmid (Oxaphenamide) is a choleretic drug, inhibits ribonucleotide reductase activity by targeting ribonucleotide reductase small subunit M2 (RRM2).
Targets&IC50	RRM2:8.23 μM
In vitro	Osalmid has been identified as a potential compound targeting the ribonucleotide reductase small subunit M2 (RRM2), exhibiting a tenfold higher efficacy in inhibiting ribonucleotide reductase (RR) activity than hydroxyurea. It significantly suppresses both HBV DNA and cccDNA synthesis within HepG2.2.15 cells, following a time- and dose-dependent manner. The effective concentration (EC50) for inhibiting HBV DNA is noted as 11.1 μM in culture supernatant and 16.5 μM in cells, following an 8-day treatment with Osalmid, which demonstrates a concentration-dependent suppression of RR activity, marked by an IC50 of 8.23 μM. Moreover, Osalmid has demonstrated potent activity against a 3TC-resistant HBV strain, indicating its potential in treating drug-resistant HBV infections[1].
In vivo	Osalmid diminishes ribonucleotide reductase (RR) activity and hepatitis B virus (HBV) replication in HBV-transgenic mice, demonstrating synergistic effectiveness with lamivudine (3TC) while maintaining a low toxicity profile. Administered orally at a dosage of 400 mg/kg/day, osalmid progressively inhibits HBV DNA replication over time. A four-week treatment regimen results in a 40-45% decrease in HBV DNA replication levels in both the sera and liver tissues of mice, in comparison to untreated controls[1].
Cell Research	HepG2.2.15 cells are cultured in the presence of 200 μg/mL G418. Cell viability is determined using a Cell Counting Kit-8 in 96-well plates treated with Osalmid for designated times. For long term assays, the culture supernatants are replaced with fresh media containing Osalmid every two days. The control wells contained equivalent amounts of DMSO. The CC50 is calculated as the concentration of a compound that reduced the cell viability to 50% compared to the control[1].
Synonyms	Oxaphenamide, 4'-Hydroxysalicylanilide

Chemical Properties

Molecular Weight	229.23
Formula	C₁₃H₁₁NO₃
Cas No.	526-18-1
Smiles	OC1=CC=C(NC(=O)C2=CC=CC=C2O)C=C1
Relative Density.	1.2084 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 247.5 mg/mL (1079.7 mM), Sonication is recommended.

Ethanol: 41 mg/mL (178.86 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.72 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.3624 mL	21.8122 mL	43.6243 mL	218.1215 mL
5 mM	0.8725 mL	4.3624 mL	8.7249 mL	43.6243 mL
10 mM	0.4362 mL	2.1812 mL	4.3624 mL	21.8122 mL
20 mM	0.2181 mL	1.0906 mL	2.1812 mL	10.9061 mL
50 mM	0.0872 mL	0.4362 mL	0.8725 mL	4.3624 mL
100 mM	0.0436 mL	0.2181 mL	0.4362 mL	2.1812 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc