Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

HCT116 cells:0.9 μM, HCT116 cells:1 μM, F0F1-ATPase:1 μM (Ki), SH-SY5Y cells:29 μM, K562 cells:0.2 μM

METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants.
RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. [1]
METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates.
RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour. [2]

METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days.
RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration. [3]

ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference)