Shopping Cart

Your shopping cart is currently empty

Nigericin

Name: Nigericin
Brand: TargetMol
SKU: T16323
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T16323Cas No. 28380-24-7

Nigericin is a polyether antibiotic with potent antimicrobial, antimalarial, anticoronaviral and anticancer activities. It is capable of promoting cellular potassium efflux, inducing Caspase-1/GSDMD and Caspase-3-mediated cellular pyroptosis and apoptosis in triple-negative breast cancer, and inducing apoptosis in PEL (primary exudative lymphoma) cells through mitochondrial dysfunction and Wnt/β-catenin signaling downregulation.

Nigericin

Copy Product Info

😃Good

Purity: 99.02%

Catalog No. T16323Cas No. 28380-24-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
5 mg	$65	In Stock	In Stock
10 mg	$111	In Stock	In Stock
25 mg	$179	In Stock	In Stock
50 mg	$239	In Stock	In Stock
100 mg	$372	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.02%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Nigericin is a polyether antibiotic with potent antimicrobial, antimalarial, anticoronaviral and anticancer activities. It is capable of promoting cellular potassium efflux, inducing Caspase-1/GSDMD and Caspase-3-mediated cellular pyroptosis and apoptosis in triple-negative breast cancer, and inducing apoptosis in PEL (primary exudative lymphoma) cells through mitochondrial dysfunction and Wnt/β-catenin signaling downregulation.
Targets&IC50	4T1 cells:2.505 μM, MDA-MB-231 cells:2.881 μM
In vitro	Methods: MDA-MB-231, MDA-MB-468, SK-BR-3, MCF-7 and 4T1 cells were treated with Nigericin (0.25, 0.5, 1, 2, 5, 10 and 20 μg/mL), cell viability was measured by MTS assay, and IC50 values were calculated based on the cell viability curves. Results: Nigericin inhibited the cell viability of MDA-MB-231 and 4T1 cells in a dose-dependent manner, with IC50 values of 2.881 μM and 2.505 μM, respectively. [4]
In vivo	Methods: BALB/c mice transplanted with 4T1 cells were treated with Nigericin (2 mg/kg, subcutaneously, every two days for 4 weeks), and tumor volume was monitored every 3 to 4 days. At the end of the experiment, mice were euthanized by carbon dioxide, and tumors were harvested for further analysis. Results: Nigericin inhibited tumor growth. [4]

Chemical Properties

Molecular Weight	724.96
Formula	C₄₀H₆₈O₁₁
Cas No.	28380-24-7
Smiles	C[C@H]1[C@]2(O[C@@](C)(C1)[C@@]3(O[C@@](C)(CC3)[C@@]4(O[C@](C[C@@H]4C)([C@]5(O[C@](CO)(O)[C@H](C)C[C@@H]5C)[H])[H])[H])[H])O[C@H](C[C@@H]6O[C@@]([C@H](C(O)=O)C)([C@@H](C)CC6)[H])C[C@@H](OC)[C@H]2C
Relative Density.	1.19 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 5 mg/mL (6.9 mM), Sonication is recommended.

Ethanol: 45.91 mg/mL (63.33 mM), Sonication is recommended.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.3794 mL	6.8969 mL	13.7939 mL	68.9693 mL
5 mM	0.2759 mL	1.3794 mL	2.7588 mL	13.7939 mL

Ethanol

	1mg	5mg	10mg	50mg
10 mM	0.1379 mL	0.6897 mL	1.3794 mL	6.8969 mL
20 mM	0.0690 mL	0.3448 mL	0.6897 mL	3.4485 mL
50 mM	0.0276 mL	0.1379 mL	0.2759 mL	1.3794 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc