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Anti-Caspase-3 Antibody (1B38) is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Antibody (1B38) can be used in IF,IHC-Fr,IHC-P,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $153 | 7-10 days | |
50 μL | $272 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Anti-Caspase-3 Antibody (1B38) is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Antibody (1B38) can be used in IF,IHC-Fr,IHC-P,WB. |
Synonyms | Yama, SCA-1, CPP32B, CPP32, apopain |
Ig Type | IgG |
Clone | 1B38 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated (TMAB-00287) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. 25 μg total protein per Lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (TMAB-00287) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 10. 25 μg total protein per Lane of various lysates probed with Caspase-3 monoclonal antibody, unconjugated (TMAB-00287) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
Application | |
Recommended Dose | IHC-Fr: 1:50-200; IHC-P: 1:50-200; IF: 1:50-200; WB: 1:500-2000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. |
Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Research Background | The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. |
Immunogen | A synthesized peptide: human Caspase 3 |
Antigen Species | Human |
Gene Name | CASP3 |
Gene ID | |
Protein Name | Caspase-3 |
Uniprot ID | |
Biology Area | Caspases,Metabolism,Caspases,Caspases,Other proteases,Apoptosis |
Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
Molecular Weight | Theoretical: 32 kDa. Actual: 12/29/32 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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