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Methacycline hydrochloride

Name: Methacycline hydrochloride
Brand: TargetMol
SKU: T0518
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0518Cas No. 3963-95-9

Alias Rondomycin, Methacycline HCl

Methacycline hydrochloride (Rondomycin) is a broad-spectrum semisynthetic antibiotic related to TETRACYCLINE but excreted more slowly and maintaining effective blood levels for a more extended period.

Methacycline hydrochloride

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Purity: 99.71%

Catalog No. T0518Alias Rondomycin, Methacycline HClCas No. 3963-95-9

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$30	In Stock	In Stock
50 mg	$38	In Stock	In Stock
100 mg	$52	In Stock	In Stock
500 mg	$129	-	In Stock
1 g	$192	-	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.71%

Color:White to Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Methacycline hydrochloride (Rondomycin) is a broad-spectrum semisynthetic antibiotic related to TETRACYCLINE but excreted more slowly and maintaining effective blood levels for a more extended period.
In vitro	Methacycline, within pulmonary alveolar macrophages, does not alter the response gene of TGF-β1 nor attenuates the aggregation of inflammatory cells. Following tracheal aspiration of bleomycin, intraperitoneal injection of Methacycline at 100 mg/kg, starting on day 10, enhances survival rates by day 17. Methacycline mitigates bleomycin-induced classical EMT (Epithelial-Mesenchymal Transition) markers, including SNAIL1, TWIST1, type I collagen, fibronectin, and their mRNA expressions.
In vivo	Methacycline inhibits the TGF-β1-induced non-Smad signaling pathways, including the activation of c-Jun N-terminal kinase (JNK), p38, and Akt, without suppressing Smad or β-catenin transcriptional activities. It does not affect the baseline activities of JNK, p38, or Akt, nor the TGF-β1 response of lung fibroblasts. Additionally, methacycline inhibits the TGF-β1-induced expression of α-smooth muscle actin (α-SMA), SNAIL1, and type I collagen in primary alveolar epithelial cells.
Synonyms	Rondomycin, Methacycline HCl

Chemical Properties

Molecular Weight	478.88
Formula	C₂₂H₂₃ClN₂O₈
Cas No.	3963-95-9
Smiles	Cl.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@@H]3C(=C)c4cccc(O)c4C(=O)C3=C(O)[C@]2(O)C(=O)C(C(N)=O)=C1O
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 27.5 mg/mL (57.43 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2 mg/mL (4.18 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0882 mL	10.4410 mL	20.8821 mL	104.4103 mL
5 mM	0.4176 mL	2.0882 mL	4.1764 mL	20.8821 mL
10 mM	0.2088 mL	1.0441 mL	2.0882 mL	10.4410 mL
20 mM	0.1044 mL	0.5221 mL	1.0441 mL	5.2205 mL
50 mM	0.0418 mL	0.2088 mL	0.4176 mL	2.0882 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc