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Lithocholic acid

🥰Excellent
Catalog No. T2202Cas No. 434-13-9
Alias 3α-Hydroxy-5β-cholanic acid

Lithocholic acid (3α-Hydroxy-5β-cholanic acid) is a toxic secondary bile acid, They were FXR antagonists (IC50=0.7, 1.4 μM), EphA2 antagonists (IC50=48, 66 μM) and EphB4 antagonists (IC50=141 μM). Lithocholic acid can promote intrahepatic cholestasis and promote tumorigenesis. Lithocholic acid is a cleanser that dissolves fat for absorption and is itself absorbed.

Lithocholic acid

Lithocholic acid

🥰Excellent
Purity: 99.93%
Catalog No. T2202Alias 3α-Hydroxy-5β-cholanic acidCas No. 434-13-9
Lithocholic acid (3α-Hydroxy-5β-cholanic acid) is a toxic secondary bile acid, They were FXR antagonists (IC50=0.7, 1.4 μM), EphA2 antagonists (IC50=48, 66 μM) and EphB4 antagonists (IC50=141 μM). Lithocholic acid can promote intrahepatic cholestasis and promote tumorigenesis. Lithocholic acid is a cleanser that dissolves fat for absorption and is itself absorbed.
Pack SizePriceAvailabilityQuantity
1 g$35In Stock
2 g$44In Stock
5 g$64In Stock
1 mL x 10 mM (in DMSO)$29In Stock
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Purity:99.93%
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Product Introduction

Bioactivity
Description
Lithocholic acid (3α-Hydroxy-5β-cholanic acid) is a toxic secondary bile acid, They were FXR antagonists (IC50=0.7, 1.4 μM), EphA2 antagonists (IC50=48, 66 μM) and EphB4 antagonists (IC50=141 μM). Lithocholic acid can promote intrahepatic cholestasis and promote tumorigenesis. Lithocholic acid is a cleanser that dissolves fat for absorption and is itself absorbed.
Targets&IC50
HCT116 cells (p53−/−):37.4 ± 3.8 μM (LD50), USP2a:31.1 ± 3.4 µM, PC-3 cells:32.0 µM, DU-145 cells:30.4 µM, HCT116 cells (wt):46.8 ± 2.6 μM (LD50)
In vitro
METHODS: Human A549, HCT-116, HT-29, Huh-7, LoVo, MGC-803, RKO, SK-HEP1, SW480 cells were treated with Lithocholic acid (200, 100, 50, P < 0.05). 25, 12.5, 6.25, 3.125, 1.5625 μM) were used to detect the cell growth inhibition by MTT method.
RESULTS: Lithocholic acid did not affect the growth of A549, HCT-116, HT-29, Huh-7, LoVo, MGC-803, RKO, SK-HEP1, and SW480 cells (IC50> 200 µM). [1]
METHODS: Human Caco2 and HT-1080 cells were treated with Lithocholic acid (0.1-1000 μM) for 24 hours, and the cytotoxicity was detected by MTT assay.
RESULTS: Lithocholic acid significantly inhibited the growth of Caco2 (IC50=56 μM) and HT-1080 cells (IC50=23 μM). [2]
METHODS: Human DLD1, HCT-116, and HCT-8 cells were treated with Lithocholic acid (25, 50, 100, 150, and 200μM) for 48 hours, and the cytotoxicity was detected by MTT assay.
RESULTS: Lithocholic acid significantly inhibited the growth of human DLD1 (IC50=173.1 μM), HCT-116 (IC50=81.1 μM) and HCT-8 (IC50=97.4 μM) cells. [3]
In vivo
METHODS: To investigate the antiviral effect of Lithocholic acid, Lithocholic acid oleate (3%) and Lithocholic acid (0.5%), derivatives of LCA, were administered to HSV-1 virus infected mice.
RESULTS: Mice treated with Lithocholic acid oleate+Lithocholic acid 48 hours before virus inoculation showed lesions on day 6 after infection, and the wound healing was better than that of the acyclovir treatment group, with reduced scar formation and better skin repair. [4]
Kinase Assay
Competitive ligand binding assay.: Ligand binding is performed using lysates from COS-7 cells transfected with expression plasmids for VDR or RXRα. Binding is performed overnight at 4°C in lysate buffer with 0.71 nM (18 Ci/mmol) [3H]1,25(OH)2D3 and bile acid competitor. Unbound [3H]1,25(OH)2D3 is removed by adsorption to dextran-coated charcoal and the supernatant removed for scintillation counting. Ki values are calculated from a computer fit of competition curves from triplicate assays.
Synonyms3α-Hydroxy-5β-cholanic acid
Chemical Properties
Molecular Weight376.57
FormulaC24H40O3
Cas No.434-13-9
Smiles[H][C@@]12CC[C@H]([C@H](C)CCC(O)=O)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])CC[C@]2([H])C[C@H](O)CC[C@]12C
Relative Density.1.0454 g/cm3 (Estimated)
Storage & Solubility Information
Storagekeep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
Ethanol: 44 mg/mL (116.84 mM), Sonication is recommended.
DMSO: 55 mg/mL (146.06 mM), Sonication is recommended.
H2O: < 1 mg/mL (insoluble or slightly soluble)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.6555 mL13.2777 mL26.5555 mL132.7774 mL
5 mM0.5311 mL2.6555 mL5.3111 mL26.5555 mL
10 mM0.2656 mL1.3278 mL2.6555 mL13.2777 mL
20 mM0.1328 mL0.6639 mL1.3278 mL6.6389 mL
50 mM0.0531 mL0.2656 mL0.5311 mL2.6555 mL
100 mM0.0266 mL0.1328 mL0.2656 mL1.3278 mL

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