L-Hisidine is an essential amino acid for infants and acts as an inhibitor of mitochondrial glutamine transport.

L-histidine notably impedes growth, with its impacts on viability inversely associated with FLO11 expression. In strains Δflo11 and S288c, this compound does not influence viability. Additionally, L-histidine substantially reduces air-liquid biofilm formation and adherence to polystyrene in flor yeasts, while not altering the transcription level of the FLO11 gene. Furthermore, it alters the content of chitin and glycans in the cell walls of flor yeasts.

Administering L-histidine (100 mg/kg) effectively prevents brain edema in rats treated with thioacetamide. In the L-histidine diet group, histamine release induced by high K+ levels in the hypothalamus is reduced to 60% compared to the control group. However, levels of other monoamines and their metabolites remain unchanged by the L-histidine diet. Behavioral assessments, such as the open-field and light/dark box tests, reveal that rats on the L-histidine diet spend less time in the central and light zones, respectively, indicating anxiety-like behaviors induced by the diet.