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GSK-J4 Hydrochloride

Name: GSK-J4 Hydrochloride
Brand: TargetMol
SKU: T4383
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4383Cas No. 1797983-09-5

Alias GSK J4 HCl (1373423-53-0 free base), GSK J4 HCl

GSK-J4 Hydrochloride (GSK J4 HCl) is a cell permeable, potent and selective histone demethylase(JMJD3 )inhibitor. is an ethyl ester derivative of the GSK-J1 with an IC50 value greater than 50 μM in vitro.

GSK-J4 Hydrochloride

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Purity: 98.23%

Catalog No. T4383Alias GSK J4 HCl (1373423-53-0 free base), GSK J4 HClCas No. 1797983-09-5

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$34	In Stock	In Stock
2 mg	$48	In Stock	In Stock
5 mg	$81	In Stock	In Stock
10 mg	$129	In Stock	In Stock
25 mg	$229	In Stock	In Stock
50 mg	$382	In Stock	In Stock
100 mg	$562	In Stock	In Stock
200 mg	$785	-	In Stock
1 mL x 10 mM (in DMSO)	$142	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.23%

Color:Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	GSK-J4 Hydrochloride (GSK J4 HCl) is a cell permeable, potent and selective histone demethylase(JMJD3 )inhibitor. is an ethyl ester derivative of the GSK-J1 with an IC50 value greater than 50 μM in vitro.
Targets&IC50	JMJD3:50 μM, JMJD3 (cell-free assay):60 nM
In vitro	When administered to human primary macrophages, GSK-J4 can reduce LPS-induced proinflammatory cytokine production, most notably including that of TNFα (IC50 = 9 μM).
In vivo	Acting as a prodrug, GSK-J4 is rapidly hydrolyzed in cells to the otherwise cell impermeable GSK-J1, which exhibits an IC50 value of 60 nM for the purified enzyme. The histone H3 lysine 27 (H3K27) demethylase JMJD3 plays important roles in the transcriptional regulation of cell differentiation, development, the inflammatory response, and cancer.
Synonyms	GSK J4 HCl (1373423-53-0 free base), GSK J4 HCl

Chemical Properties

Molecular Weight	453.97
Formula	C₂₄H₂₈ClN₅O₂
Cas No.	1797983-09-5
Smiles	Cl.CCOC(=O)CCNc1cc(nc(n1)-c1ccccn1)N1CCc2ccccc2CC1
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 20 mg/mL (44.06 mM), Sonication is recommended.

DMSO: 50 mg/mL (110.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.41 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2028 mL	11.0139 mL	22.0279 mL	110.1394 mL
5 mM	0.4406 mL	2.2028 mL	4.4056 mL	22.0279 mL
10 mM	0.2203 mL	1.1014 mL	2.2028 mL	11.0139 mL
20 mM	0.1101 mL	0.5507 mL	1.1014 mL	5.5070 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0441 mL	0.2203 mL	0.4406 mL	2.2028 mL
100 mM	0.0220 mL	0.1101 mL	0.2203 mL	1.1014 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc