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Crystal Violet

Name: Crystal Violet
Brand: TargetMol
SKU: T1343L
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1343LCas No. 548-62-9

Alias Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3

Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.

Crystal Violet

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Purity: 99.6%

Catalog No. T1343LAlias Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3Cas No. 548-62-9

Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.

Pack Size	Price	USA Warehouse	Global Warehouse
5 g	$33	-	In Stock
10 g	$42	-	In Stock
25 g	$67	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.6%

Appearance:Solid

Color:Green to Cyan

Resource Download

COA HNMR LCMS

Product Introduction

Crystal Violet AI Summary

Crystal Violet exhibits a broad spectrum of bioactivities, notably showing potent trypanocidal effects against the trypomastigote form of Trypanosoma cruzi. It has demonstrated significant efficacy in eliminating parasites with an ED50 value of 536.0 µM or 100.0 µg/mL and IC50 values ranging between 7.5 µg/mL to 536,000.0 nM under different conditions. The compound was noted for achieving 100.0% parasite lysis at a concentration of 250 µg/mL. Additionally, it displays antimicrobial activity against a variety of bacterial strains including Escherichia coli and Salmonella serovar Typhimurium, with MIC values ranging from 0.5 µg/mL to 4096.0 µg/mL. The compound also shows potential therapeutic relevance through inhibition of SARS-CoV-2-induced cytotoxicity, with reported inhibition of cytotoxicity induced by the virus in VERO-6 cells. Moreover, Crystal Violet possesses cytotoxic effects against several mammalian cell lines, including BALB/c mouse splenocytes and human HeLa cells, as well as anti-inflammatory activity by reducing nitric oxide production in mouse RAW264.7 cells. The diverse bioactivities of Crystal Violet reflect its potential for therapeutic applications against parasitic infections, bacterial diseases, viral infections, and inflammatory conditions..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.
Cell Research	Usage method a. Solution preparation: Dissolve 2 g Crystal Violet in 20 mL 95% ethanol solution and mix with 80 mL 1% ammonium oxalate aqueous solution, and use after 24 hours. b. Operation steps: 1. Sample processing 1) Paraffin sections: First use xylene to dewax for 5-10 min, then use fresh xylene and dewax for another 5-10 min. After dewaxing, use 100% ethanol for 5 min; then use 90% ethanol for 2 min; finally use 70% ethanol for 2 min; ddH2O for 2 min. 2) Frozen sections: distilled water for 2 min. 3) Tissue culture cells: Fix with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, then use fresh distilled water and wash again for 2 min. 2. Crystal violet staining: After the sample is treated by the above method, add crystal violet staining solution directly and stain at room temperature for 10 minutes (the staining solution needs to cover the sample, and the specific staining time can be adjusted according to the desired staining results). 3. Pour out the staining solution and wash thoroughly with distilled water until no dye is washed off. Dry at room temperature. 4. Observe and take pictures under an optical microscope. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3

Chemical Properties

Molecular Weight	407.98
Formula	C₂₅H₃₀ClN₃
Cas No.	548-62-9
Smiles	[Cl-].CN(C)c1ccc(cc1)C(\c1ccc(cc1)N(C)C)=C1\C=C/C(/C=C\1)=[N+](/C)C
Relative Density.	0.471 g/cm3.

Storage & Solubility Information

Storage

keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 8 mg/mL (19.61 mM), Sonication is recommended.

H2O: 12 mg/mL (29.41 mM), Sonication is recommended.

DMSO: 76 mg/mL (186.28 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (8.09 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4511 mL	12.2555 mL	24.5110 mL	122.5550 mL
5 mM	0.4902 mL	2.4511 mL	4.9022 mL	24.5110 mL
10 mM	0.2451 mL	1.2256 mL	2.4511 mL	12.2555 mL

H2O/DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1226 mL	0.6128 mL	1.2256 mL	6.1278 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0490 mL	0.2451 mL	0.4902 mL	2.4511 mL
100 mM	0.0245 mL	0.1226 mL	0.2451 mL	1.2256 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc