Leucocrystal Violet is a triphenylmethane dye.

Instructions
I. Reagent preparation: Dissolve it in an appropriate solvent (such as water or buffer solution). Depending on the experimental requirements, the common concentration range is 0.1-1 mM.
II. Operation steps
1. Sample preparation: The sample can be water, soil, air or biological fluids. If the sample contains impurities or other metal ions, it may need to be pretreated or diluted to reduce interference.
2. Reaction steps:
1) Take a certain volume of sample solution (such as environmental water samples, biological fluids, etc.) and add Leucocrystal Violet solution.
2) Usually, the reaction is carried out under acidic conditions, and the pH value of the solution is adjusted to the optimal range of the reaction using an acid of appropriate concentration (such as hydrochloric acid, acetic acid, etc.).
3) The sample reacts with Leucocrystal Violet at room temperature for a certain time (usually 10-30 minutes) to form a purple complex.
3. Measurement:
1) Use a spectrophotometer to measure the absorbance (OD value) at an appropriate wavelength (usually 590 nm). The concentration of the purple complex generated after the reaction of antimony and LCV is proportional to the absorbance.
2) According to the standard curve, calculate the concentration of antimony in the sample.
4. Data analysis:
1) According to the absorbance value of the sample, the concentration of antimony is estimated through the standard curve.
2) Blank control experiments and comparisons between samples may be required to ensure the accuracy of the results.
Notes:
1. Interfering substances: Other metal ions (such as lead, chromium, etc.) in the sample may react with Leucocrystal Violet and affect the results. Therefore, appropriate separation or suppression of interference from other metals may be required before the experiment.
2. pH control: The pH value of the reaction has an important influence on sensitivity and stability. The pH value of the reaction system should be ensured to be within the optimal range.
3. Stability: The purple complex after the reaction should be measured as soon as possible, because the stability of the complex is poor under light or high temperature and may degrade.