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AFMK

Name: AFMK
Brand: TargetMol
SKU: T41345
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T41345Cas No. 52450-38-1

Alias Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamine

AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is an active metabolite of Melatonin with antioxidant and free radical scavenging activity. AFMK is a modulator of apoptosis and improves the anti-tumor effect of Gemcitabine in PANC-1 cells.

AFMK

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Purity: 99.81%

Catalog No. T41345Alias Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamineCas No. 52450-38-1

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$40	-	In Stock
5 mg	$73	-	In Stock
10 mg	$113	-	In Stock
25 mg	$189	-	In Stock
50 mg	$283	-	In Stock
100 mg	$419	-	In Stock
1 mL x 10 mM (in DMSO)	$83	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.81%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is an active metabolite of Melatonin with antioxidant and free radical scavenging activity. AFMK is a modulator of apoptosis and improves the anti-tumor effect of Gemcitabine in PANC-1 cells.
Targets&IC50	AhR:44 μM (EC50), PPARγ:44 μM (EC50)
In vitro	In PANC-1 cell, AFMK in combination with Gemcitabine increases the inhibition of the production of HSP70 from 0.47 (Gemcitabine alone) to 0.13 (10 nM AFMK), 0.08 (0.1 nM AFMK) and 0.01 (0.001 nM AFMK)[1]. AFMK pretreatment significantly inhibits DNA damage and shows a very high hydroxyl radical scavenging potential with an IC50 of 338.08 nM[3].
In vivo	In male C57BL mice, AFMK (10 mg/kg; i.p.) significantly reverses radiation-induced decline in the total antioxidant capacity of plasma. Pretreatment of AFMK shows a significantly lower value of comet tail length and % DNA in tail[3].
Synonyms	Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamine

Chemical Properties

Molecular Weight	264.28
Formula	C₁₃H₁₆N₂O₄
Cas No.	52450-38-1
Smiles	COc1ccc(NC=O)c(c1)C(=O)CCNC(C)=O

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (189.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.57 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7839 mL	18.9193 mL	37.8387 mL	189.1933 mL
5 mM	0.7568 mL	3.7839 mL	7.5677 mL	37.8387 mL
10 mM	0.3784 mL	1.8919 mL	3.7839 mL	18.9193 mL
20 mM	0.1892 mL	0.9460 mL	1.8919 mL	9.4597 mL
50 mM	0.0757 mL	0.3784 mL	0.7568 mL	3.7839 mL
100 mM	0.0378 mL	0.1892 mL	0.3784 mL	1.8919 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc