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AFMK

Name: AFMK
Brand: TargetMol
SKU: T41345
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamine) Copy Product Info

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Synonyms: Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamine

Catalog No. T41345 Copy Product Info

Purity: 99.81%

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AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is a natural product and an apoptosis modulator with antioxidant and free radical scavenging activities. This compound enhances the antitumor effect of gemcitabine in PANC-1 cells and attenuates X-ray-induced oxidative damage to DNA, proteins, and lipids.

Cas No. 52450-38-1

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Pack Size	Price	USA Stock	Global Stock
2 mg	$40	In Stock	In Stock
5 mg	$73	In Stock	In Stock
10 mg	$113	In Stock	In Stock
25 mg	$189	In Stock	In Stock
50 mg	$283	In Stock	In Stock
100 mg	$419	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$83	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.81%

Appearance:Solid

Color:Yellow

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	AFMK (Formyl-N-acetyl-5-methoxykynurenamine) is a natural product and an apoptosis modulator with antioxidant and free radical scavenging activities. This compound enhances the antitumor effect of gemcitabine in PANC-1 cells and attenuates X-ray-induced oxidative damage to DNA, proteins, and lipids.
Targets & IC50	AhR:44 μM (EC50), PPARγ:44 μM (EC50)
In vitro	Methods: Normal human epidermal keratinocytes were used as a model. AFMK was dissolved in ethanol and pre-incubated at concentrations of 10⁻³–10⁻⁹ M for 1 h, followed by 25 mJ/cm² UVB irradiation and 48 h culture. MTT, ATP, mitochondrial membrane potential, cytochrome c release, Caspase 3/7, and sub-G1 apoptosis assays were performed. Results: AFMK significantly enhanced cell viability, maintained ATP and membrane potential, inhibited cytochrome c release, caspase activation, and apoptosis, exerting mitochondrial protective effects. [1] Methods: Rat pancreatic acinar AR42J cells were used. Cells were incubated with 10⁻¹²–10⁻⁸ M AFMK combined with 10⁻⁸ M caerulein stimulation for 48 h. Western blot was used to detect GPx, TNF-α, Bcl-2, Bax, and Caspase-3 protein expression. Results: AFMK dose-dependently increased GPx, Bax, and Caspase-3 levels, decreased TNF-α and Bcl-2 levels, and exerted protective effects by regulating antioxidant and apoptosis pathways.[2]
In vivo	Methods: In male Wistar rats, an acute pancreatitis model was induced by subcutaneous infusion of caerulein. AFMK (5, 10, 20 mg/kg) was administered by single intraperitoneal injection 30 min before modeling, with solvent being saline plus absolute ethanol; the control group received an equal volume of solvent. Results: AFMK dose-dependently alleviated pancreatic tissue edema, reduced serum amylase and TNF-α levels, validating its in vivo anti-inflammatory and antioxidant activities.[3]
Synonyms	Formyl-N-acetyl-5-methoxykynurenamine, Acetyl-N-formyl-5-methoxykynurenamine

Chemical Properties

Molecular Weight	264.28
Formula	C₁₃H₁₆N₂O₄
Cas No.	52450-38-1
Smiles	COc1ccc(NC=O)c(c1)C(=O)CCNC(C)=O

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (189.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.57 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7839 mL	18.9193 mL	37.8387 mL	189.1933 mL
5 mM	0.7568 mL	3.7839 mL	7.5677 mL	37.8387 mL
10 mM	0.3784 mL	1.8919 mL	3.7839 mL	18.9193 mL
20 mM	0.1892 mL	0.9460 mL	1.8919 mL	9.4597 mL
50 mM	0.0757 mL	0.3784 mL	0.7568 mL	3.7839 mL
100 mM	0.0378 mL	0.1892 mL	0.3784 mL	1.8919 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.