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AAPK-25

Name: AAPK-25
Brand: TargetMol
SKU: T10215
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10215Cas No. 2247919-28-2

AAPK-25 is a potent and selective Aurora/PLK dual inhibitor with anti-tumor activity, causing mitotic delay and arresting cells in prometaphase, followed by increased apoptosis. It targets Aurora-A/-B/-C with Kd values ranging from 23-289 nM.

AAPK-25

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Purity: 97.05%

Catalog No. T10215Cas No. 2247919-28-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$52	In Stock	In Stock
5 mg	$127	In Stock	In Stock
10 mg	$191	In Stock	In Stock
25 mg	$347	In Stock	In Stock
50 mg	$521	In Stock	In Stock
100 mg	$756	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$140	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.05%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	AAPK-25 is a potent and selective Aurora/PLK dual inhibitor with anti-tumor activity, causing mitotic delay and arresting cells in prometaphase, followed by increased apoptosis. It targets Aurora-A/-B/-C with Kd values ranging from 23-289 nM.
Targets&IC50	PI3K:7.11 μM (kd), PLK2:272 nM (kd), Aurora B:78 nM (kd), PLK3:456 nM (kd), Aurora A:23 nM (kd), ERK:5.32 μM (kd), Aurora C:289 nM (kd), CDK:8.02 μM , PLK1:55 nM (kd)
In vitro	AAPK-25 inhibits the growth of HCT-116, Calu6, A549, and MCF-7 cells (IC50s: 0.4, 5.3, 11.6, and 2.3 μM). It induces apoptosis in a dose-dependent manner in the HCT-116 cell line, significantly increases histone H3Ser10 phosphorylation, indicating a marked mitotic block, and notably inhibits the mitotic spindle checkpoint.
In vivo	AAPK-25 enhances the survival rate in the BALB/c nude mice tumor xenograft model.

Chemical Properties

Molecular Weight	442.32
Formula	C₂₁H₁₃Cl₂N₃O₂S
Cas No.	2247919-28-2
Smiles	Clc1ccc(cc1Cl)C(=O)Nc1ccc2cc(ccc2c1)C(=O)Nc1nccs1
Relative Density.	1.536 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (113.04 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2608 mL	11.3040 mL	22.6081 mL	113.0403 mL
5 mM	0.4522 mL	2.2608 mL	4.5216 mL	22.6081 mL
10 mM	0.2261 mL	1.1304 mL	2.2608 mL	11.3040 mL
20 mM	0.1130 mL	0.5652 mL	1.1304 mL	5.6520 mL
50 mM	0.0452 mL	0.2261 mL	0.4522 mL	2.2608 mL
100 mM	0.0226 mL	0.1130 mL	0.2261 mL	1.1304 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc