Shopping Cart

Remove All
Your shopping cart is currently empty

AAPK-25

Name: AAPK-25
Brand: TargetMol
SKU: T10215
Price: 87 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T10215Cas No. 2247919-28-2

AAPK-25 is a potent and selective Aurora/PLK dual inhibitor with anti-tumor activity, causing mitotic delay and arresting cells in prometaphase, followed by increased apoptosis. It targets Aurora-A/-B/-C with Kd values ranging from 23-289 nM.

AAPK-25

🥰Excellent

Purity: 97.05%

Catalog No. T10215Cas No. 2247919-28-2

Pack Size	Price	Availability
1 mg	$87	In Stock
5 mg	$212	In Stock
10 mg	$318	In Stock
25 mg	$579	In Stock
50 mg	$868	In Stock
100 mg	$1,260	In Stock
1 mL x 10 mM (in DMSO)	$245	In Stock

Add to Cart

Add to Quotation

Bulk & Custom

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:97.05%

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	AAPK-25 is a potent and selective Aurora/PLK dual inhibitor with anti-tumor activity, causing mitotic delay and arresting cells in prometaphase, followed by increased apoptosis. It targets Aurora-A/-B/-C with Kd values ranging from 23-289 nM.
Targets&IC50	Aurora A:23 nM (kd), Aurora C:289 nM (kd), ERK:5.32 μM (kd), Aurora B:78 nM (kd), PLK3:456 nM (kd), PI3K:7.11 μM (kd), PLK2:272 nM (kd), CDK:8.02 μM , PLK1:55 nM (kd)
In vitro	AAPK-25 inhibits the growth of HCT-116, Calu6, A549, and MCF-7 cells (IC50s: 0.4, 5.3, 11.6, and 2.3 μM). It induces apoptosis in a dose-dependent manner in the HCT-116 cell line, significantly increases histone H3Ser10 phosphorylation, indicating a marked mitotic block, and notably inhibits the mitotic spindle checkpoint.
In vivo	AAPK-25 enhances the survival rate in the BALB/c nude mice tumor xenograft model.

Chemical Properties

Molecular Weight	442.32
Formula	C₂₁H₁₃Cl₂N₃O₂S
Cas No.	2247919-28-2
Smiles	Clc1ccc(cc1Cl)C(=O)Nc1ccc2cc(ccc2c1)C(=O)Nc1nccs1
Relative Density.	1.536 g/cm3 (Predicted)
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (113.04 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2608 mL	11.3040 mL	22.6081 mL	113.0403 mL
5 mM	0.4522 mL	2.2608 mL	4.5216 mL	22.6081 mL
10 mM	0.2261 mL	1.1304 mL	2.2608 mL	11.3040 mL
20 mM	0.1130 mL	0.5652 mL	1.1304 mL	5.6520 mL
50 mM	0.0452 mL	0.2261 mL	0.4522 mL	2.2608 mL
100 mM	0.0226 mL	0.1130 mL	0.2261 mL	1.1304 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc