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Anti-Aurora B Antibody (9I737)
😃Good
Catalog No. TMAB-00174
Anti-Aurora B Antibody (9I737) is a Rabbit antibody targeting Aurora B. Anti-Aurora B Antibody (9I737) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Aurora B Antibody (9I737)
Anti-Aurora B Antibody (9I737)
😃Good
Catalog No. TMAB-00174
Anti-Aurora B Antibody (9I737) is a Rabbit antibody targeting Aurora B. Anti-Aurora B Antibody (9I737) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $272 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human,Mouse |
| Ig Type | IgG |
| Clone | 9I737 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Sample: Lane 1: Human Hela cell Lysates Lane 2: Human 293T cell Lysates Primary: Anti-Aurora B (TMAB-00174) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa 2. Blocking buffer: 5% NFDM/TBST Primary dilution: 1:1000 Primary incubation condition: 2 hours at room temperature Secondary: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: 293, 3: A20 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 39 kDa Observed MW: 39 kDa 3. Cell line: A549 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary dilution: 1:50 Primary incubation condition: 4°C overnight Secondary: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-00174 4. Tissue: Human tonsil Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-00174 5. Tissue: Mouse spleen Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-00174 |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:200-1000; IHC-P: 1:20-100; IHC-Fr: 1:400-800; ICC/IF: 1:20-50; IF: 1:20-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromsomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]. |
| Immunogen | KLH conjugated synthetic peptide: human Aurora B |
| Antigen Species | Human |
| Gene ID | 9212 |
| Uniprot ID | |
| Biology Area | Aurora,Aurora,Phosphorylation,Aurora |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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