Anti-Aurora B Antibody (9I737) is a Rabbit antibody targeting Aurora B. Anti-Aurora B Antibody (9I737) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

9I737

Human,Mouse

1. Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Aurora B (TMAB-00174) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:1000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293, 3: A20
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 39 kDa
Observed MW: 39 kDa
3. Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-00174
4. Tissue: Human tonsil
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00174
5. Tissue: Mouse spleen
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00174

WB: 1:200-1000; IHC-P: 1:20-100; IHC-Fr: 1:400-800; ICC/IF: 1:20-50; IF: 1:20-50

Monoclonal

KLH conjugated synthetic peptide: human Aurora B

Human

AURKB

Aurora kinase B

Aurora,Aurora,Phosphorylation,Aurora

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis. A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity.