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SAG hydrochloride (912545-86-9(free base))

Catalog No. T4211 CAS 2095432-58-7

Synonyms: Smoothened Agonist HCl

SAG hydrochloride (912545-86-9(free base)) (Smoothened Agonist HCl) acts as an SMO agonist.

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SAG hydrochloride (912545-86-9(free base)) Chemical Structure

SAG hydrochloride (912545-86-9(free base)), CAS 2095432-58-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 47.00
5 mg	In stock	$ 97.00
10 mg	In stock	$ 155.00
25 mg	In stock	$ 289.00
50 mg	In stock	$ 396.00
100 mg	In stock	$ 593.00
1 mL * 10 mM (in DMSO)	In stock	$ 113.00

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Purity: 99.19%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	SAG hydrochloride (912545-86-9(free base)) (Smoothened Agonist HCl) acts as an SMO agonist.
In vitro	SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. [1] SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. [2] SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells.
In vivo	In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. [3] In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities.
Animal Research	Animal Models: RatFormulation: DMSODosages: 2.5 nMAdministration: Intracerebroventricular administration(Only for Reference)

Synonyms	Smoothened Agonist HCl
Molecular Weight	526.52
Formula	C28H29Cl2N3OS
CAS No.	2095432-58-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 38 mg/mL (72.2 mM)

DMSO: 66 mg/mL (125.4 mM)

References and Literature

1. Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6. 2. Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6. 3. Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13. 4. Fish EW, et al. Preaxial polydactyly following early gestational exposure to the smoothened agonist, SAG, in C57BL/6J mice. Birth Defects Res 2017 Jan 20;109(1):49-54. .

Citations

1. Wu H, Zhang L, Chen B, et al.B13, a well-tolerated inhibitor of hedgehog pathway, exhibited potent anti-tumor effects against colorectal carcinoma in vitro and in vivo.Bioorganic Chemistry.2023: 106488.

Related compound libraries

This product is contained In the following compound libraries：

Membrane Protein-targeted Compound Library GPCR Compound Library Bioactive Compound Library Wnt/Hedgehog/Notch Compound Library Anti-Aging Compound Library Bioactive Compounds Library Max Stem Cell Differentiation Compound Library Osteogenesis Compound Library Reprogramming Compound Library NO PAINS Compound Library

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Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SAG hydrochloride (912545-86-9(free base)) 2095432-58-7 GPCR/G Protein Stem Cells Hedgehog/Smoothened SAG hydrochloride (912545 86 9(free base)) Smoothened Agonist HCl SAG hydrochloride (912545869(free base)) inhibitor inhibit

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