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Rostafuroxin

Catalog No. T2621 CAS 156722-18-8

Synonyms: PST 2238

Rostafuroxin has been used in trials studying the treatment of Essential Hypertension.

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Rostafuroxin, CAS 156722-18-8

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Rostafuroxin has been used in trials studying the treatment of Essential Hypertension.
Targets&IC50	Na+-ATPase:1.5 nM, K+-ATPase:1.5 nM
Kinase Assay	Kinase assay: Activity assays are conducted using Lance Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) technology from Perkin-Elmer. Briefly, 10 ng/mL FLT3 enzyme, a serial diluted G-749, 80 nM substrate of ULight-poly-GT peptide and variable amounts of ATP (8.5 μM to 1088 μM) are mixed in kinase assay buffer (50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT and 0.01% Tween-20) and are added to a 384-well OptiPlate-384 in a volume of 10 μL. Kinase reactions are incubated at room temperature for up to 1 h and then stopped by the addition of 5 μL of 10 mM EDTA. A volume of 5 μL of the specific Eu-labeled-anti-phosphopeptide antibody diluted in LANCE Detection Buffer is then added to a final concentration of 2 nM. After 30-minute incubation, assay plates are incubated at 23°C and the LANCE signal is measured on an EnVision Multilabel Reader. Excitation wavelength is set at 320 nm and emission monitored at 615 nm (donor) and 665 nm (acceptor). The IC50 is calculated using nonlinear regression analysis analysis by GradPad Prism 5.

Synonyms	PST 2238
Molecular Weight	374.521
Formula	C23H34O4
CAS No.	156722-18-8

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 10 mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Wenceslau CF, Rossoni LV. J Hypertens. 2014 Mar; 32(3): 542-54. 2. Gan H, Qi M, Chan C, et al. Digitoxin inhibits HeLa cell growth through the induction of G2/M cell cycle arrest and apoptosis in vitro and in vivo[J]. International journal of oncology . 2020.

Citations

1. Gan H, Qi M, Chan C, et al. Digitoxin inhibits HeLa cell growth through the induction of G2/M cell cycle arrest and apoptosis in vitro and in vivo. International journal of oncology. 2020

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Anti-Cancer Metabolism Compound Library Anti-Cardiovascular Disease Compound Library Inhibitor Library Ion Channel Inhibitor Library Drug Repurposing Compound Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Target-Focused Phenotypic Screening Library Cardiotoxicity Compound Library

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Keywords

Rostafuroxin 156722-18-8 离子通道微生物学 ATPase RSV ATP1A1 PST-2238 Respiratory syncytial virus Na+/K+ ATPase phosphorylation inhibit PST2238 Tyr845 Sodium potassium pump EGFR antihypertensive RSV-GFP Inhibitor digitoxigenin PST 2238 Ouabain inhibitor

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