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RBC8

Catalog No. T6634 CAS 361185-42-4

RBC8 is a specific GTPases RalA/RalB inhibitor by inhibiting the binding of Ral to its effector RALBP1, no inhibition on the GTPases RhoA and Ras.

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RBC8, CAS 361185-42-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 45.00
2 mg	In stock	$ 62.00
5 mg	In stock	$ 111.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 333.00
50 mg	In stock	$ 497.00
100 mg	In stock	$ 723.00
1 mL * 10 mM (in DMSO)	In stock	$ 118.00

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Purity: 99.81%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	RBC8 is a specific GTPases RalA/RalB inhibitor by inhibiting the binding of Ral to its effector RALBP1, no inhibition on the GTPases RhoA and Ras.
Targets&IC50	Ral GTPase (H358 cells):3.4 μM, GTPase (H2122 cells):3.5 μM
In vitro	In mice bearing H358 xenografts, RBC8 administered intraperitoneally (i.p.) at a dosage of 50 mg/kg specifically inhibits RalA and RalB, thereby suppressing tumor growth.
In vivo	Within living cells, RBC8k can induce a chemical shift change in RalB-GDP, thereby diminishing the activation of RalA. In Ral-dependent H2122 (IC50: 3.5 μM) and H358 (IC50: 3.4 μM) models, RBC8 leads to the inhibition of anchorage-independent growth.
Cell Research	Growth inhibition of human lung cancer cells by the compounds is measured under anchorage-independent conditions in soft agar. Cells are seeded into 6-well plates (coated with a base layer made of 2.0 ml of 1% low-melting-point agarose) at 15,000 cells per well in 3.0 mL of 0.4% low-melting-point agarose containing various concentration of drug. Two to four weeks (depending on the cell line) after incubation, the cells are stained with 1.0 mg ml?1 nitroblue tetrazolium, and colonies are counted under a microscope. The IC50 values are defined as the concentration of drug that resulted in a 50% reduction in colony number compared with the DMSO-treated control. (Only for Reference)

Molecular Weight	424.45
Formula	C25H20N4O3
CAS No.	361185-42-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 42.4 mg/mL (100 mM)

References and Literature

1. Yan C, et al. Nature. 2014, 515(7527), 443-447. 2. Wang P, Zhang W, Wang L, et al. RCC2 Interacts with Small GTPase RalA and Regulates Cell Proliferation and Motility in Gastric Cancer[J]. OncoTargets and therapy. 2020, 13: 3093.

Citations

1. Wang P, Zhang W, Wang L, et al. RCC2 Interacts with Small GTPase RalA and Regulates Cell Proliferation and Motility in Gastric Cancer. OncoTargets and Therapy. 2020, 13: 3093

Related compound libraries

This product is contained In the following compound libraries：

Reprogramming Compound Library Anti-Colorectal Cancer Compound Library MAPK Inhibitor Library Anti-Prostate Cancer Compound Library Kinase Inhibitor Library Inhibitor Library GPCR Compound Library Autophagy Compound Library Ferroptosis Compound Library HIF-1 Signaling Pathway Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

RBC8 361185-42-4 GPCR/G Protein MAPK GTPase Ras Inhibitor RBC-8 inhibit RBC 8 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.