Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Pifithrin-α is a p53 inhibitor, inhibiting p53-dependent transactivation of p53-responsive genes.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 33.00 | |
5 mg | In stock | $ 52.00 | |
10 mg | In stock | $ 81.00 | |
25 mg | In stock | $ 180.00 | |
50 mg | In stock | $ 293.00 | |
100 mg | In stock | $ 438.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 54.00 |
Description | Pifithrin-α is a p53 inhibitor, inhibiting p53-dependent transactivation of p53-responsive genes. |
Kinase Assay | The ligand binding competition assays are performed. Cytosolic cell extracts from Hepa-1 cells are generated by the resuspension of the cell pellets in HEDG buffer [25 mM Hepes, 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol, pH 7.5] containing 0.4 mM leupeptin, 4 mg/mL aprotinin, and 0.3 mM phenylmethylsulfonyl fluoride, homogenization, and centrifugation at 100,000 g for 45 min. Aliquots of the supernatant (120 μg) are incubated at room temperature for 2 h with the indicated concentrations of Pifithrin-α in the presence of 3 nM [3H]TCDD in HEDG buffer. After incubation on ice with hydroxyapatite for 30 min, HEDG buffer with 0.5% Tween 80 is added. The samples are centrifuged, washed twice, resuspended in 0.2 mL of scintillation fluid, and subjected to scintillation counting. Nonspecific binding is determined using a 150-fold molar excess of TCDF and subtracted from the total binding to obtain the specific binding. The specific binding is reported relative to [3H]TCDD alone[2]. |
Cell Research | At the end of cell treatments, the number of attached cells is estimated by staining with 0.25% crystal violet in 50% methanol, followed by elution of the dye with 1% SDS. Optical density (530 nm) reflecting the number of stained cells is determined with a Bio-Tek EL311 microplate reader. Cell viability in suspension of short term culture of primary thymocytes is determined by their staining with 0.1% of methyl blue and microscopic counting of blue (dead) cells.(Only for Reference) |
Synonyms | Pifithrin hydrobromide, PFTα hydrobromide, PFTα, Pifithrin-α, Pifithrin-α (PFTα) HBr |
Molecular Weight | 367.3 |
Formula | C16H18N2OS·HBr |
CAS No. | 63208-82-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
DMSO: 36.7 mg/mL (100 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Pifithrin-α hydrobromide 63208-82-2 Apoptosis Immunology/Inflammation p53 Aryl Hydrocarbon Receptor Ferroptosis inhibit Pifithrin α hydrobromide Inhibitor MDM-2/p53 AhR Pifithrin Pifithrin hydrobromide PFTα hydrobromide PFTα Pifithrin-α Pifithrin-α (PFTα) HBr Pifithrinα hydrobromide inhibitor