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Neogambogic acid

Catalog No. T5724 CAS 93772-31-7

Synonyms: neo-gambogic acid

Neogambogic acid, an active ingredient in garcinia, can inhibit the growth of some solid tumors and result in an anticancer effect, it may be responsible for the inhibition of proliferation of human breast cancer cell line MCF-7 cells.

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Neogambogic acid, CAS 93772-31-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 46.00
5 mg	In stock	$ 98.00
10 mg	In stock	$ 148.00
25 mg	In stock	$ 249.00
50 mg	In stock	$ 369.00
100 mg	In stock	$ 548.00
1 mL * 10 mM (in DMSO)	In stock	$ 160.00

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Purity: 99.38%

Purity: 97.74%

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Description	Neogambogic acid, an active ingredient in garcinia, can inhibit the growth of some solid tumors and result in an anticancer effect, it may be responsible for the inhibition of proliferation of human breast cancer cell line MCF-7 cells.
In vitro	Neo-gambogic acid(NGA) significantly reduced the proliferation of MCF-7 cells in a dose-dependent manner. NGA could increase the expression of the apoptosis-related proteins FasL, caspase-3, caspase-8, caspase-9, and Bax and decrease the expression of anti-apoptotic protein Bcl-2 accompanied by the mitochondrial transmembrane damage. The antiproliferative effect of NGA on MCF-7 cells is due to the G(0)/G(1) arrest, increased apoptosis and activation of Fas/FasL and cytochrome C pathway[1].
In vivo	Neo-gambogic acid(NGA) dose of ≤0.4 μg/ml had no significant effect on the proliferation of mouse BMMs in vitro (P>0.05);?concentrations of between 0.1-0.4 μg/ml significantly inhibited RANKL-induced osteoclastogenesis (P<0.01) in a dose-dependent manner.?Compared with the control group, NGA significantly reduced RANKL-induced bone resorption in vitro (P <0.01), and downregulated the expression of osteoclast-related mRNAs of TRAP, CTR, CTSK, and NFATc1.?NGA suppressed the activation of JNK but not the p38 signaling pathway and significantly reduced NF-κB p65 phosphorylation and the nuclear transport of NF-κB molecules, which inhibited NFATc1 expression.?CONCLUSIONS NGA suppressed RANKL-induced osteoclastogenesis by inhibiting the JNK and NF-κB pathways in mouse BMMs in vitro and reduced osteoclastic bone resorption[2].
Cell Research	MCF-7 cells (5* 10^4 ) were seeded into 96-well plates.Four hours later, 10 ul NGA in DMSO was added into the wells at various concentrations (0.5-24 ug/ml) and 0.1% DMSO was set as a negative control.?After 72 h, 50 ul MTT was added and cells were incubated for another 4 h. Then, media was removed and 150 ul DMSO was added and the plates were placed on a shaking table at 150 rpm for 10 min. Optical density (OD) was measured at 490 nm.?The experiment was repeated thrice and the rate of cell inhibition was calculated using the following formula: inhibition rate [1-(OD test/ OD negative control)]*100%[1].
Animal Research	Primary mouse BMMs were cultured with increasing concentrations of NGA.?Real-time polymerase chain reaction was used to study the expression of mRNAs corresponding to gene products specific to receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation, including tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), cathepsin K (CTSK), and nuclear factor of activated T cells c1 (NFATc1).?A cell counting kit-8 assay was used to evaluate cell proliferation.?Western blotting and confocal immunofluorescence microscopy were used to investigate the signaling pathways.?A bone resorption model was used to quantify bone resorption[2].
Source	Natural Products > Clusiaceae > Garcinia

Synonyms	neo-gambogic acid
Molecular Weight	646.77
Formula	C38H46O9
CAS No.	93772-31-7

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 6.47 mg/mL (10 mM)

References and Literature

1. Wang K, Tang Y, Sun M,et al.The mechanism of neogambogic acid-induced apoptosis in human MCF-7 cells[J].Acta Biochim Biophys Sin (Shanghai). 2011 Sep;43(9):698-702. 2. Jin G , Wang F F , Li T , et al. Neogambogic Acid Suppresses Receptor Activator of Nuclear Factor κB Ligand (RANKL)-Induced Osteoclastogenesis by Inhibiting the JNK and NF-κB Pathways in Mouse Bone Marrow-Derived Monocyte/Macrophages[J]. Medical Science Monitor International Medical Journal of Experimental & Clinical Research, 2018, 24:2569-2577.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Selected Plant-Sourced Compound Library Anti-infective Natural Product Library Anti-Infection Compound Library NO PAINS Compound Library Bioactive Compounds Library Max Natural Product Library for HTS Bioactive Compound Library Anti-Bacterial Compound Library Anti-Cancer Compound Library

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Keywords

Neogambogic acid 93772-31-7 Apoptosis Microbiology/Virology Others Antibacterial cancer MRSA neo-gambogic acid arrest breast MCF-7 Bacterial Inhibitor inhibit tummor cells proliferation inhibitor

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