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Mdivi-1

Catalog No. T1907 CAS 338967-87-6

Synonyms: Mitochondrial division inhibitor 1

Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.

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Mdivi-1, CAS 338967-87-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 39.00
10 mg	In stock	$ 51.00
25 mg	In stock	$ 92.00
50 mg	In stock	$ 155.00
100 mg	In stock	$ 239.00
200 mg	In stock	$ 355.00
500 mg	In stock	$ 588.00
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

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Purity: 98.79%

Purity: 98.64%

Purity: 98.32%

Purity: 98.24%

Purity: 98.22%

Purity: 98.072%

Purity: 98%

Purity: 96.57%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.
Targets&IC50	Dnm1 GTPase:1 μM-10 μM
In vitro	In the ganglion cell layer (GCL), Mdivi-1 exhibits strong immunoreactivity. Twelve hours following ischemic induction, Mdivi-1 significantly increases protein expression within the GCL. It notably reduces the expression of GFAP protein without altering Drp1 protein expression. In the normal murine retina, Mdivi-1 primarily localizes to the inner plexiform layer, ganglion cell layer, outer plexiform layer, and inner nuclear layer. Early in the development of the ischemic murine retina, there is a marked increase in the protein expression of Mdivi-1 and glial fibrillary acidic protein (GFAP). Mdivi-1 inhibits apoptosis in the ischemic retina and significantly increases the survival rate of retinal ganglion cells (RGC) two weeks post-ischemia.
In vivo	Mdivi-1 inhibits the ATPase activity and self-assembly of Dnm1 by inducing a conformational change (IC50<10 μM). It effectively suppresses C8-Bid and STS-induced mitochondrial outer membrane permeabilization (MOMP) in HeLa cells and extracellular mouse liver mitochondria. Mdivi-1 blocks the divisionof Dynamin-related GTPases, yeast Dnm1, and human Drp1, facilitating efficient and reversible mitochondrial fusion into a net-like structure. Intracellularly, it prevents apoptosis by inhibiting mitochondrial outer membrane permeabilization. Mdivi-1 represents a potential therapeutic class for treating stroke, myocardial infarction, and neurodegenerative diseases.
Kinase Assay	All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.
Cell Research	Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.

Synonyms	Mitochondrial division inhibitor 1
Molecular Weight	353.22
Formula	C15H10Cl2N2O2S
CAS No.	338967-87-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 35.3 mg/mL (100 mM)

References and Literature

1. Fang CT, et al. Mdivi-1 induces spindle abnormalities and augments taxol cytotoxicity in MDA-MB-231 cells. Cell Death Discov. 2021 May 20;7(1):118. 2. Dai W, et al. Mitochondrial division inhibitor (mdivi-1) decreases oxidative metabolism in cancer. Br J Cancer. 2020 Apr;122(9):1288-1297. 3. Park SW, et al. A selective inhibitor of drp1, mdivi-1, increases retinal ganglion cell survival in acute ischemic mouse retina. Invest Ophthalmol Vis Sci. 2011 Apr 27;52(5):2837-43. 4. Li YH, et al. Mdivi-1, a mitochondrial fission inhibitor, modulates T helper cells and suppresses the development of experimental autoimmune encephalomyelitis. J Neuroinflammation. 2019 Jul 19;16(1):149.

Citations

1. Li Q, Chu Y, Li S, et al. The oncoprotein MUC1 facilitates breast cancer progression by promoting Pink1-dependent mitophagy via ATAD3A destabilization. Cell Death & Disease. 2022, 13(10): 1-16. 2. Guo Q, Zhang Y C, Wang W, et al. Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. Pharmacological Research. 2022: 106046. 3. Guo Q, Zhang Y C, Wang W, et al. Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. Pharmacological Research. 2022: 106046. 4. Li C, Pan Y, Tan Y, et al. PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity. Frontiers in cell and developmental biology. 2022, 10. 5. Shi Y, Liu Q, Chen W, et al. Protection of Taohong Siwu Decoction on PC12 cells injured by oxygen glucose deprivation/reperfusion via mitophagy-NLRP3 inflammasome pathway in vitro. Journal of Ethnopharmacology. 2022: 115784. 6. Wang J, Su Q, Wu Q, et al. Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells. Archives of Pharmacal Research. 2021: 1-12. 7. Su Q, Wang J, Liu F, et al. Blocking Parkin/PINK1-mediated mitophagy sensitizes hepatocellular carcinoma cells to sanguinarine-induced mitochondrial apoptosis. Toxicology in Vitro. 2020: 104840 8. Su Q, Wu Q, Chen K, et al. Induction of Estrogen Receptor β-mediated Autophagy Sensitizes Breast Cancer Cells to TAD1822-7, a Novel Biphenyl Urea Taspine Derivative. Molecular Biology Reports. 2021 9. Ma L, Chen C, Hai S, et al.Inhibition of Mitochondrial Fission Alleviates Zearalenone-Induced Mitochondria-Associated Endoplasmic Reticulum Membrane Dysfunction in Piglet Sertoli Cells.Toxins.2023, 15(4): 253. 10. Wang H, Ye J, Peng Y, et al.CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction.Autophagy.2023 (just-accepted).

11. Wang Y, He X, Xue M, et al.Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy.Free Radical Research.2023 (just-accepted): 1-36. 12. Guo Y, Zhu L, Duan Y, et al.Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission.Cell Death & Disease.2024, 15(2): 125. 13. Fu Q, Zhang Y B, Shi C X, et al.GSDMD/Drp1 signaling pathway mediates hippocampal synaptic damage and neural oscillation abnormalities in a mouse model of sepsis-associated encephalopathy.Journal of Neuroinflammation.2024, 21(1): 1-16.

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Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Cuproptosis Compound Library Anti-Aging Compound Library Bioactive Compounds Library Max NO PAINS Compound Library Bioactive Compound Library Autophagy Compound Library Apoptosis Compound Library Covalent Inhibitor Library Cytoskeletal Signaling Pathway Compound Library

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Keywords

Mdivi-1 338967-87-6 Apoptosis Autophagy Cytoskeletal Signaling Mitophagy Dynamin Mitochondrial division inhibitor 1 Mitochondrial Autophagy Mitochondrial division inhibitor1 Inhibitor inhibit Mdivi 1 Mdivi1 Mitochondrial division inhibitor-1 inhibitor

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