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FMK 9a

Catalog No. T15303 CAS 1955550-51-2

FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.

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FMK 9a, CAS 1955550-51-2

Pack Size	Availability	Price/USD
1 mg	In stock	$ 142.00
5 mg	In stock	$ 347.00
10 mg	In stock	$ 519.00
25 mg	In stock	$ 842.00
50 mg	In stock	$ 1,160.00
100 mg	In stock	$ 1,570.00
500 mg	In stock	$ 3,130.00
1 mL * 10 mM (in DMSO)	In stock	$ 298.00

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Purity: 98.97%

ee: 100%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.
Targets&IC50	Autophagin-1 (FRET assay):80 nM, Autophagin-1 (LRA assay):73 nM
In vitro	FMK 9a forms a covalent bond with the more reactive thiol group of Cys74 located at the catalytic site of ATG4B thereby inactivating ATG4B proteolytic activity[1].
In vivo	FMK 9a shows moderate solubility and high liver microsome clearances for human and mice of 13.9 and 70 mL/kg/min[1].

Molecular Weight	392.42
Formula	C23H21FN2O3
CAS No.	1955550-51-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 95 mg/mL (242.09 mM), Sonication is recommended.

References and Literature

1. Qiu Z, et al. Discovery of Fluoromethylketone-Based Peptidomimetics as Covalent ATG4B (Autophagin-1) Inhibitors. ACS Med Chem Lett. 2016 Jun 25;7(8):802-6.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max Autophagy Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

FMK 9a 1955550-51-2 Autophagy Proteases/Proteasome Cysteine Protease inhibit Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.