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Crystal Violet

🥰Excellent
Catalog No. T1343LCas No. 548-62-9
Alias Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3

Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.

Crystal Violet

Crystal Violet

🥰Excellent
Catalog No. T1343LAlias Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3Cas No. 548-62-9
Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.
Pack SizePriceAvailabilityQuantity
500 mg$46In Stock
5 g$51In Stock
10 g$55In Stock
1 mL x 10 mM (in DMSO)$51In Stock
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Product Introduction

Bioactivity
Description
Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.
Cell Research
Usage method
a. Solution preparation: Dissolve 2 g Crystal Violet in 20 mL 95% ethanol solution and mix with 80 mL 1% ammonium oxalate aqueous solution, and use after 24 hours.
b. Operation steps:
1. Sample processing
1) Paraffin sections: First use xylene to dewax for 5-10 min, then use fresh xylene and dewax for another 5-10 min. After dewaxing, use 100% ethanol for 5 min; then use 90% ethanol for 2 min; finally use 70% ethanol for 2 min; ddH2O for 2 min.
2) Frozen sections: distilled water for 2 min.
3) Tissue culture cells: Fix with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, then use fresh distilled water and wash again for 2 min.
2. Crystal violet staining: After the sample is treated by the above method, add crystal violet staining solution directly and stain at room temperature for 10 minutes (the staining solution needs to cover the sample, and the specific staining time can be adjusted according to the desired staining results).
3. Pour out the staining solution and wash thoroughly with distilled water until no dye is washed off. Dry at room temperature.
4. Observe and take pictures under an optical microscope.
AliasMethyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3
Chemical Properties
Molecular Weight407.98
FormulaC25H30ClN3
Cas No.548-62-9
Smiles[Cl-].CN(C)c1ccc(cc1)C(\c1ccc(cc1)N(C)C)=C1\C=C/C(/C=C\1)=[N+](/C)C
Relative Density.0.471 g/cm3.
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
Ethanol: 8 mg/mL (19.61 mM), Sonication is recommended.
H2O: 12 mg/mL (29.41 mM), Sonication is recommended.
DMSO: 76 mg/mL (186.28 mM), Sonication is recommended.
Solution Preparation Table
Ethanol/H2O/DMSO
1mg5mg10mg50mg
1 mM2.4511 mL12.2555 mL24.5110 mL122.5550 mL
5 mM0.4902 mL2.4511 mL4.9022 mL24.5110 mL
10 mM0.2451 mL1.2256 mL2.4511 mL12.2555 mL
H2O/DMSO
1mg5mg10mg50mg
20 mM0.1226 mL0.6128 mL1.2256 mL6.1278 mL
DMSO
1mg5mg10mg50mg
50 mM0.0490 mL0.2451 mL0.4902 mL2.4511 mL
100 mM0.0245 mL0.1226 mL0.2451 mL1.2256 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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