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Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
500 mg | $46 | In Stock | |
5 g | $51 | In Stock | |
10 g | $55 | In Stock | |
1 mL x 10 mM (in DMSO) | $51 | In Stock |
Description | Crystal Violet (Hexamethylpararosaniline chloride) is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties. |
Cell Research | Usage method a. Solution preparation: Dissolve 2 g Crystal Violet in 20 mL 95% ethanol solution and mix with 80 mL 1% ammonium oxalate aqueous solution, and use after 24 hours. b. Operation steps: 1. Sample processing 1) Paraffin sections: First use xylene to dewax for 5-10 min, then use fresh xylene and dewax for another 5-10 min. After dewaxing, use 100% ethanol for 5 min; then use 90% ethanol for 2 min; finally use 70% ethanol for 2 min; ddH2O for 2 min. 2) Frozen sections: distilled water for 2 min. 3) Tissue culture cells: Fix with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, then use fresh distilled water and wash again for 2 min. 2. Crystal violet staining: After the sample is treated by the above method, add crystal violet staining solution directly and stain at room temperature for 10 minutes (the staining solution needs to cover the sample, and the specific staining time can be adjusted according to the desired staining results). 3. Pour out the staining solution and wash thoroughly with distilled water until no dye is washed off. Dry at room temperature. 4. Observe and take pictures under an optical microscope. |
Alias | Methyl Violet 10B, Hexamethylpararosaniline chloride, Gentian Violet, Basic Violet 3 |
Molecular Weight | 407.98 |
Formula | C25H30ClN3 |
Cas No. | 548-62-9 |
Smiles | [Cl-].CN(C)c1ccc(cc1)C(\c1ccc(cc1)N(C)C)=C1\C=C/C(/C=C\1)=[N+](/C)C |
Relative Density. | 0.471 g/cm3. |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||||||||||||||||||||||||||||||||
Solubility Information | Ethanol: 8 mg/mL (19.61 mM), Sonication is recommended. H2O: 12 mg/mL (29.41 mM), Sonication is recommended. DMSO: 76 mg/mL (186.28 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||||||||||||
Ethanol/H2O/DMSO
H2O/DMSO
DMSO
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