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Apelin-13

Catalog No. T7656 CAS 217082-58-1

Apelin-13 is a 13-amino acid peptide sequence found in the central and peripheral nervous system that is an endogenous ligand for the G protein-coupled receptor angiotensin II protein J (APJ), with an EC50 value of 0.37 nM for APJ.Apelin-13 has vasodilatory and antihypertensive activity and can be used to study type 2 diabetic syndrome. Apelin-13 has vasodilatory and antihypertensive activities and can be used to study type 2 diabetes syndrome.

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Apelin-13, CAS 217082-58-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 47.00
5 mg	In stock	$ 150.00
10 mg	In stock	$ 257.00
25 mg	In stock	$ 463.00
50 mg	In stock	$ 694.00

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Purity: 96.81%

Biological Description

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Storage & Solubility Information

Description	Apelin-13 is a 13-amino acid peptide sequence found in the central and peripheral nervous system that is an endogenous ligand for the G protein-coupled receptor angiotensin II protein J (APJ), with an EC50 value of 0.37 nM for APJ.Apelin-13 has vasodilatory and antihypertensive activity and can be used to study type 2 diabetic syndrome. Apelin-13 has vasodilatory and antihypertensive activities and can be used to study type 2 diabetes syndrome.
Targets&IC50	APJ:0.37 nM
In vitro	In cultured PC12 cells, the same protective effects were also observed.?Silencing Nrf2 gene with its siRNA abolished the Apelin 13's prevention of I/R-induced PC12 cell injury, oxidative stress, and inflammation.?Inhibition of AMPK by its siRNA decreased the level of Apelin 13-induced Nrf2 expression and diminished the protective effects of Apelin 13.?The interplay relationship between GSK-3β and Nrf2 was also verified with relative overexpression.?Using selective inhibitors, we further identified the upstream of AMPK/GSK-3β/Nrf2 is AR/Gα/PLC/IP3/CaMKK.
In vivo	Administered intraperitoneally at a daily dosage of 200 µg/kg for 4 weeks, Apelin-13 improves cardiac function, insulin resistance, and lipid metabolism. It significantly decreases serum levels of TNF-α and leptin, induces the expression of Apelin-12 in serum, and markedly elevates levels of GLUT4 and p-AMPKα2[2]. When delivered via intracerebroventricular injection at single dosages of 10 and 100 μM, Apelin-13 increases the spontaneous discharges in the majority of pallidal neurons[3].
Cell Research	For in vitro study, PC12 cells were used,proteases inhibitors or siRNA were used.?Protein levels were investigated by western blotting.
Animal Research	In vivo, the I/R model was induced and Apelin 13 was given intracerebroventricularly 15 min before reperfusion.?The neurobehavioral scores, infarction volumes, and some cytokines in the brain were measured.

Molecular Weight	1550.83
Formula	C69H111N23O16S
CAS No.	217082-58-1

Storage

store at low temperature,keep away from moisture

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 50 mg/mL (32.24 mM）, Sonication is recommended.

DMSO: 50 mg/mL (32.24 mM), Sonication is recommended.

References and Literature

1. Tatemoto, K., Hosoya, M., Habata, Y., et al. Isolation and characterizaton of a novel endogenous peptide ligand for the human APJ receptor. Biochemical and Biophysical Research Communications 251, 471-476 (1998). 2. Li M,et al. Apelin 13 ameliorates metabolic and cardiovascular disorders in a rat model of type 2 diabetes with a high fat diet. Mol Med Rep. 2018 Dec;18(6):5784-5790. 3. Wang Y, et al. Apelin-13 regulates electrical activity in the globus pallidus and induces postural changes in rats. Neural Regen Res. 2021 Nov;16(11):2264-2268. 4. Yamaleyeva LM, et al. Apelin-13 in blood pressure regulation and cardiovascular disease. Curr Opin Nephrol Hypertens. 2016 Sep;25(5):396-403.

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Keywords

Apelin-13 217082-58-1 inhibit Inhibitor Apelin Receptor (APJ) nervous systems antihypertensive metabolic syndrome Apelin13 diabetes Goto-Kakizaki rat insulin resistance pallidal neurons lipid Apelin 13 Wistar rat vasodilatory inhibitor

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