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Immunology/Inflammation COX Acetaminophen

Acetaminophen

Catalog No. T0065   CAS 103-90-2
Synonyms: 4'-Hydroxyacetanilide, 4-Acetamidophenol, APAP, Paracetamol

Analgesic antipyretic derivative of acetanilide. Acetaminophen has weak anti-inflammatory properties and is used as a common analgesic, but may cause liver, blood cell, and kidney damage.

Acetaminophen, CAS 103-90-2
Pack Size Availability Price/USD Quantity
5 mg In stock 16.00
10 mg In stock 22.00
25 mg In stock 28.00
50 mg In stock 35.00
100 mg In stock 42.00
200 mg In stock 50.00
500 mg In stock 60.00
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Purity 99.85%
Purity 99.99%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Analgesic antipyretic derivative of acetanilide. Acetaminophen has weak anti-inflammatory properties and is used as a common analgesic, but may cause liver, blood cell, and kidney damage.
Targets&IC50 COX-1 ,   COX-2
Kinase Assay Effect of inhibition of Acetaminophen on COX-1 and COX-2 activity in human whole blood: For COX-1 assay, aliquots of human whole blood drawn from healthy volunteers without anticoagulant are transferred to glass tubes containing Acetaminophen or DMSO, serum is separated by centrifugation after clotting, and serum TxB2 levels are determined. For COX-2 assay, aliquots of heparinized whole blood are incubated with LPS (10 μg/mL) and aspirin (10 μg/mL), plus Acetaminophen or DMSO for 24 hours at 37 °C, plasma is separated by centrifugation, and PGE2 levels are determined subsequently. The degree of COX-1 or COX-2 inhibition is calculated as the percentage change of plasma eicosanoid (TxB2 for COX-1 and PGE2 for COX-2).Concentration response curves are fitted by a sigmoidal regression with variable slope for both enzymatic assays, and the 50% inhibitory concentration (IC50) values are derived by using of PRISM Version 3.0.
Cell Research
Cells are exposed to Acetaminophen for 48 hours. Cell viability is determined by the trypan blue exclusion method. Intracellular GSH is measured by recording the disulfide, GS-TNB and 5-thio-nitrobenzoic acid (TNB), the yellow colored compound formed by the reaction between GSH with DTNB.(Only for Reference)
Cell lines: SK-MEL-28, SK-MEL-5, MeWo, Saos-2, SW-620, PC-3, B16-F0, B16-F10, BJ fibroblast cell lines and Human epidermal melanocytes
Synonyms 4'-Hydroxyacetanilide , 4-Acetamidophenol , APAP , Paracetamol
Purity 99.85%
Molecular Weight 151.16
Formula C8H9NO2
CAS No. 103-90-2

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

Water: Soluble

DMSO: 15.1 mg/mL (100 mM)

Ethanol: 15.1 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Hinz B, et al. FASEB J, 2008, 22(2), 383-390. 2. Vad NM, et al. J Pharm Sci, 2009, 98(4), 1409-1425. 3. Simmons DL, et al. Proc Natl Acad Sci, 1999, 96(6), 3275-3280. 4. Qiu Y, et al. J Biol Chem, 1998, 273(28), 17940-17953. 5. Gardner CR, et al. Hepatology, 1998, 27(3), 748-754. 6. Dini M, et al. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment ofAcetaminophen Hepatotoxicity. Front Microbiol. 2017 Apr 6;8:594. 7. Uchida NS, et al. Hepatoprotective Effect of Citral on Acetaminophen-Induced Liver Toxicity in Mice. Evid Based Complement Alternat Med. 2017;2017:1796209.

Related compound libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Natural Compound Library for HTS Anti-cancer Compound Library Clinical Compound Library Neuronal Signaling Compound Library Human Endogenous Metabolite Compound Library FDA-approved Drug Library Immunology/Inflammation Compound Library Anti-cancer Approved drug Library Anti-cancer Clinical Compound Library Anti-Cardiovascular Disease Compound Library Killers Collection CNS-Penetrant Compound Library Anti-aging Compound Library Antitumor Natural Products Library Anti-cancer Drug library Drug Repurposing Library HIF-1 Signaling Pathway Compound Library Ferroptosis Compound Library Drug-induced Liver Injury (DILI) Compound Library Nonsteroidal Antiinflammatory Compound Library FDA Approved & Pharmacopeial Drug Library Anti-Breast Cancer Compound Library Anti-fibrosis Compound Library Gut Microbial Metabolite Library

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