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Acetaminophen

Catalog No. T0065 CAS 103-90-2

Synonyms: 4-Acetamidophenol, 4'-Hydroxyacetanilide, APAP, Paracetamol

Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.

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Acetaminophen, CAS 103-90-2

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Purity: 100%

Purity: 99.99%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Acetaminophen (APAP) is a COX inhibitor that inhibits COX-1 and COX-2 (IC50=113.7/25.8 μM). Acetaminophen has antipyretic and analgesic activity as well as weak anti-inflammatory activity.
Targets&IC50	COX-2:5.8 μM
In vitro	METHODS: Non-melanoma and melanoma cell lines were treated with Acetaminophen (100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Acetaminophen showed considerable toxicity in melanoma cell lines SK-MEL-5, MeWo, B16-F0 and B16-F10, resulting in 40±3%, 45±7%, 66±8% and 60±5% cytotoxicity, respectively. Acetaminophen showed negligible toxicity at 100 μM in the non-melanoma cell lines PC-3, BJ, Saos-2 and SW-620 cells. [1] METHODS: Neuroblastoma cells SH-SY5Y were treated with Acetaminophen (2 mM) for 24-48 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Acetaminophen induced cytochrome c release from mitochondria in a time-dependent manner, reaching a maximum level after 48 h of treatment. In addition, immunoblot analysis of cytoplasmic and mitochondrial fractions showed that Acetaminophen was able to induce the accumulation of Bax into mitochondria at 24 h after treatment. [2]
In vivo	METHODS: To detect hepatotoxicity in vivo, Acetaminophen was administered intraperitoneally to mice (300 mg/kg) and rats (1 g/kg). RESULTS: Extensive liver necrosis was observed in mice, but little damage was observed in rat samples. The rats were highly resistant to Acetaminophen-induced liver injury. [3] METHODS: To test for in vivo hepatotoxicity, Acetaminophen was administered to aged and weakened mice acutely (300 mg/kg by gavage), chronically (100 mg/kg by diet once daily for six weeks), or subacutely (250 mg/kg by gavage three times daily for three days). RESULTS: There was no overall increase in Acetaminophen hepatotoxicity with age or frailty in mice, despite changes in certain pathways that would be expected to affect susceptibility to Acetaminophen toxicity. [4]
Kinase Assay	Effect of inhibition of Acetaminophen on COX-1 and COX-2 activity in human whole blood: For COX-1 assay, aliquots of human whole blood drawn from healthy volunteers without anticoagulant are transferred to glass tubes containing Acetaminophen or DMSO, serum is separated by centrifugation after clotting, and serum TxB2 levels are determined. For COX-2 assay, aliquots of heparinized whole blood are incubated with LPS (10 μg/mL) and aspirin (10 μg/mL), plus Acetaminophen or DMSO for 24 hours at 37 °C, plasma is separated by centrifugation, and PGE2 levels are determined subsequently. The degree of COX-1 or COX-2 inhibition is calculated as the percentage change of plasma eicosanoid (TxB2 for COX-1 and PGE2 for COX-2).Concentration response curves are fitted by a sigmoidal regression with variable slope for both enzymatic assays, and the 50% inhibitory concentration (IC50) values are derived by using of PRISM Version 3.0.
Cell Research	Cells are exposed to Acetaminophen for 48 hours. Cell viability is determined by the trypan blue exclusion method. Intracellular GSH is measured by recording the disulfide, GS-TNB and 5-thio-nitrobenzoic acid (TNB), the yellow colored compound formed by the reaction between GSH with DTNB.(Only for Reference)

Synonyms	4-Acetamidophenol, 4'-Hydroxyacetanilide, APAP, Paracetamol
Molecular Weight	151.16
Formula	C8H9NO2
CAS No.	103-90-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 15.1 mg/mL (100 mM)

H2O: 20mg/ml( 132.3mM), Sonification is recommended

Ethanol: 15.1 mg/mL (100 mM)

References and Literature

1. Vad NM, et al. Biochemical mechanism of acetaminophen (APAP) induced toxicity in melanoma cell lines. J Pharm Sci. 2009 Apr;98(4):1409-25. 2. Posadas I, et al. Acetaminophen induces human neuroblastoma cell death through NFKB activation. PLoS One. 2012;7(11):e50160. 3. McGill MR, et al. Acetaminophen-induced liver injury in rats and mice: comparison of protein adducts, mitochondrial dysfunction, and oxidative stress in the mechanism of toxicity. Toxicol Appl Pharmacol. 2012 Nov 1;264(3):387-94. 4. Kane AE, et al. Acetaminophen hepatotoxicity in mice: Effect of age, frailty and exposure type. Exp Gerontol. 2016 Jan;73:95-106. 5. Gardner CR, et al. Hepatology, 1998, 27(3), 748-754. 6. Dini M, et al. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment ofAcetaminophen Hepatotoxicity. Front Microbiol. 2017 Apr 6;8:594. 7. Uchida NS, et al. Hepatoprotective Effect of Citral on Acetaminophen-Induced Liver Toxicity in Mice. Evid Based Complement Alternat Med. 2017;2017:1796209.

Citations

1. Ma R, Fang L, Chen L, et al. Ferroptotic stress promotes macrophages against intracellular bacteria. Theranostics. 2022, 12(5): 2266 2. Guo J, Xu Y, Chen L J, et al. Gut Microbiota and Host Cyp450s Co-contribute to Pharmacokinetic Variability in Mice With Non-Alcoholic Steatohepatitis: Vary From Drug to Drug. Journal of Advanced Research. 2021

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Inhibitor Library Drug Repurposing Compound Library Epigenetics Compound Library Nonsteroidal Anti-Inflammatory Compound Library Drug-induced Liver Injury (DILI) Compound Library Pyroptosis Compound Library Bioactive Compounds Library Max

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Keywords

Acetaminophen 103-90-2 Chromatin/Epigenetic Immunology/Inflammation Metabolism Neuroscience COX Endogenous Metabolite Histone Acetyltransferase HATs HAT Cyclooxygenase 4-Acetamidophenol Inhibitor 4'-Hydroxyacetanilide APAP Paracetamol inhibit inhibitor

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