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4EGI-1

Name: 4EGI-1
Brand: TargetMol
SKU: T2665
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2665Cas No. 315706-13-9

Alias 4EGI 1

4EGI-1, a competitive eIF4E/eIF4 g interaction inhibitor, binds to eIF4E(KD=25 μM).

4EGI-1

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Purity: 99.12%

Catalog No. T2665Alias 4EGI 1Cas No. 315706-13-9

4EGI-1, a competitive eIF4E/eIF4 g interaction inhibitor, binds to eIF4E(KD=25 μM).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$37	In Stock	In Stock
2 mg	$50	In Stock	In Stock
5 mg	$90	In Stock	In Stock
10 mg	$117	In Stock	In Stock
25 mg	$231	In Stock	In Stock
50 mg	$369	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$98	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.12%

Color:White to Yellow

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	4EGI-1, a competitive eIF4E/eIF4 g interaction inhibitor, binds to eIF4E(KD=25 μM).
Targets&IC50	eIF4E-eIF4G:25 μM(Kd)
In vivo	In Jurkat cells, 4EGI-1 promotes apoptosis. It effectively inhibits cell growth in A549 lung cancer cells (IC50=6 μM). In human lung cancer cells, 4EGI-1 enhances TRAIL-induced apoptosis by inducing DR5 and downregulating c-FLIP, thereby inhibiting cap-dependent protein translation.
Cell Research	Cell viability is measured by treatment of Jurkat cells with compound for 24 h and by determination of intracellular ATP using the CellTiterGlo assay. For measurement of apoptotic DNA fragmentation, cells are treated for 24 h with 60 μM EGI-1 or 6.65 μM camptothecin in the presence or absence of 100 mM zVAD-FMK, a broad-spectrum caspase inhibitor. After fixation and staining with PI, cellular DNA content is determined by FACS analysis in a FACS Calibur machine. Nuclear morphology after 24 h EG1-1 treatment is visualized by staining of cells with Hoechst dye and ?uorescence microscopy. For the A549 lung cancer cells, cell growth in the presence of 4EGI-1 is determined using the SRB staining method.(Only for Reference)
Synonyms	4EGI 1

Chemical Properties

Molecular Weight	451.28
Formula	C₁₈H₁₂Cl₂N₄O₄S
Cas No.	315706-13-9
Smiles	OC(=O)C(\Cc1ccccc1[N+]([O-])=O)=N\Nc1nc(cs1)-c1ccc(Cl)c(Cl)c1
Relative Density.	1.58 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (221.59 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2159 mL	11.0796 mL	22.1592 mL	110.7960 mL
5 mM	0.4432 mL	2.2159 mL	4.4318 mL	22.1592 mL
10 mM	0.2216 mL	1.1080 mL	2.2159 mL	11.0796 mL
20 mM	0.1108 mL	0.5540 mL	1.1080 mL	5.5398 mL
50 mM	0.0443 mL	0.2216 mL	0.4432 mL	2.2159 mL
100 mM	0.0222 mL	0.1108 mL	0.2216 mL	1.1080 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc