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3BDO

Name: 3BDO
Brand: TargetMol
SKU: T14033
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14033Cas No. 890405-51-3

3BDO is a new mTOR activator. 3BDO inhibits autophagy.

3BDO

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Purity: 97.76%

Catalog No. T14033Cas No. 890405-51-3

3BDO is a new mTOR activator. 3BDO inhibits autophagy.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
2 mg	$39	In Stock	In Stock
5 mg	$62	In Stock	In Stock
10 mg	$98	In Stock	In Stock
25 mg	$217	In Stock	In Stock
50 mg	$347	In Stock	In Stock
100 mg	$519	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$68	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.76%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	3BDO is a new mTOR activator. 3BDO inhibits autophagy.
In vitro	Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced by rapamycin and inhibits its effect in HUVECs. Phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses this phosphorylation. These results demonstrate that 3BDO suppresses the increased MAP1LC3B puncta, MAP1LC3B-II levels, and decreased SQSTM1 protein levels induced by rapamycin. Additionally, 3BDO decreases FLJ11812 levels in HUVECs in a dose- and time-dependent manner, while overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO[1].
In vivo	Immunofluorescence assay demonstrates that treatment with 3BDO enhances p-p70S6K levels while reducing ATG13 protein levels in the plaque endothelium of mice, without affecting the phosphorylation of mTOR's immediate downstream targets p70S6K and 4EBP1. Comparatively, in apoE-/- mice, 3BDO treatment notably inhibits endothelium autophagy and apoptosis, thereby offering protection against endothelial injury in atherosclerosis. Furthermore, 3BDO treatment contributes to the stabilization of established atherosclerotic lesions in apoE-/- mice and results in a significant reduction in the serum levels of IL-6 and IL-8.

Chemical Properties

Molecular Weight	327.33
Formula	C₁₈H₁₇NO₅
Cas No.	890405-51-3
Smiles	[O-][N+](=O)c1ccccc1OCC1CC(Cc2ccccc2)C(=O)O1
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (458.25 mM), Sonication is recommended.

Ethanol: 20 mg/mL (61.1 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (12.22 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0550 mL	15.2751 mL	30.5502 mL	152.7510 mL
5 mM	0.6110 mL	3.0550 mL	6.1100 mL	30.5502 mL
10 mM	0.3055 mL	1.5275 mL	3.0550 mL	15.2751 mL
20 mM	0.1528 mL	0.7638 mL	1.5275 mL	7.6376 mL
50 mM	0.0611 mL	0.3055 mL	0.6110 mL	3.0550 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0306 mL	0.1528 mL	0.3055 mL	1.5275 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc