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2',7'-Dichlorofluorescein diacetate

Catalog No. T20684 CAS 2044-85-1

Synonyms: Diacetyldichlorofluorescein, DCFDA, Dcfh-DA, Leuco-dcf diacetate

2',7'-Dichlorofluorescein diacetate, a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and Reactive Oxygen Species (ROS) and for the determination of the degree of overall oxidative stress.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

2',7'-Dichlorofluorescein diacetate Chemical Structure

2',7'-Dichlorofluorescein diacetate, CAS 2044-85-1

Pack Size	Availability	Price/USD
50 mg	In stock	$ 41.00
100 mg	In stock	$ 53.00
200 mg	In stock	$ 77.00
500 mg	In stock	$ 131.00
1 mL * 10 mM (in DMSO)	In stock	$ 45.00

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Purity: 99.08%

Purity: 96.52%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

2',7'-Dichlorofluorescein diacetate, a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

Synonyms	Diacetyldichlorofluorescein, DCFDA, Dcfh-DA, Leuco-dcf diacetate
Molecular Weight	485.26
Formula	C24H14Cl2O7
CAS No.	2044-85-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 2 years

Solubility Information

DMSO: 5.79 mg/ml (11.93 mM), Need ultrasonic

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Kaur M, Esau L. Two-step protocol for preparing adherent cells for high-throughput flow cytometry. Biotechniques. 2015 Sep 1;59(3):119-26. doi: 10.2144/000114325. eCollection 2015 Sep. PubMed PMID: 26345504. 2. Aranda A, Sequedo L, Tolosa L, Quintas G, Burello E, Castell JV, Gombau L. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63. doi: 10.1016/j.tiv.2013.01.016. Epub 2013 Jan 26. PubMed PMID: 23357416. 3. Mohan S, Thiagarajan K, Chandrasekaran R. In vitro evaluation of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB. Nat Prod Res. 2015;29(4):366-9. doi: 10.1080/14786419.2014.942303. Epub 2014 Aug 7. PubMed PMID: 25104086. 4. Nanda SS, An SS, Yi DK. Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif. Int J Nanomedicine. 2015 Aug 25;10 Spec Iss:93-9. doi: 10.2147/IJN.S88378. eCollection 2015. PubMed PMID: 26347475; PubMed Central PMCID: PMC4554416.

Related compound libraries

This product is contained In the following compound libraries：

Human Metabolite Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

2',7'-Dichlorofluorescein diacetate 2044-85-1 Others oxidative stress 2',7'Dichlorofluorescein diacetate inhibit Diacetyldichlorofluorescein cell-permeable Inhibitor ROS 2′,7′-Dichlorofluorescein diacetate reactive oxygen DCFDA Dcfh-DA DCFH2-DA Leuco-dcf diacetate 2',7' Dichlorofluorescein diacetate inhibitor

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