SEMA5A Protein, Human, Recombinant (His)

Name: SEMA5A Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01570
Price: 108 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: serpin peptidase inhibitor, clade F, semF, SEMAF) Copy Product Info

Synonyms: serpin peptidase inhibitor, clade F, semF, SEMAF

Catalog No. TMPY-01570 Copy Product Info

SEMA5A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 107 kDa and the accession number is Q13591.

Pack Size	Price	USA Stock	Global Stock
5 µg	$108	-	In Stock
10 µg	$178	7-10 days	7-10 days
20 µg	$296	7-10 days	7-10 days
50 µg	$600	7-10 days	7-10 days
100 µg	$987	7-10 days	7-10 days
200 µg	$1,730	7-10 days	7-10 days
500 µg	$3,380	7-10 days	7-10 days
1 mg	$6,830	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:＞90 % as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SEMA5A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 107 kDa and the accession number is Q13591.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q13591
Construction	A DNA sequence encoding the human SEMA5A (NP_003957.2) extracellular domain (Met 1-Met 968) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 23
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	serpin peptidase inhibitor, clade F, semF, SEMAF
Research Background	Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 2 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein that belongs to the semaphorin family. Semaphorin5A / SEMA5A contains one PSI domain, one Sema domain and seven TSP type-1 domains. It may act as positive axonal guidance cues. Semaphorin5A / SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A / SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.

Chemical Properties

Molecular Weight

107 kDa (predicted); 130-140 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.