MERS-CoV Spike/RBD Protein fragment (aa 367-606, rFc)

Catalog No. TMPY-03228

Synonyms: coronavirus s2 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, RBD Prote, coronavirus spike Protein, MERS-CoV, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

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MERS-CoV Spike/RBD Protein fragment (aa 367-606, rFc)

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 904.00

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DATASHEET SDS

Biological Description

Technical Params

Product Properties

References and Literature

Description

Species	MERS-CoV
Expression System	Baculovirus-Insect Cells
Tag	rFc
Accession Number	K0BRG7
Synonyms	coronavirus s2 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, RBD Prote, coronavirus spike Protein, MERS-CoV, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV
Construction	A DNA sequence encoding the receptor binding domain (RBD) of MERS-CoV spike protein fragment (AFS88936.1) (Glu367-Tyr606) was fused with the Fc region of rabbit IgG at the C-terminus.
Protein Purity	> 80 % as determined by SDS-PAGE
Molecular Weight	51.5 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.2. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

References and Literature

1. Xiao X,et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30. 2. Shen S,et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. 3. Du L,et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.

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Keywords

MERS-CoV Spike/RBD Protein fragment (aa 367-606, rFc) coronavirus s2 Protein, MERS-CoV ncov RBD Protein, MERS-CoV cov spike Protein, MERS-CoV coronavirus s1 Protein, MERS-CoV ncov s1 Protein, MERS-CoV RBD Prote coronavirus spike Protein, MERS-CoV ncov spike Protein, MERS-CoV ncov s2 Protein, MERS-CoV recombinant recombinant-proteins proteins protein