HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein, Human, MHC (His & Avi)

HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.

Pack Size	Availability	Price/USD	Quantity
100 μg	Inquiry	$ 146.00
1 mg	Inquiry	$ 979.00

Pack Size

Availability

Price/USD

Quantity

100 μg

Inquiry

$ 146.00

1 mg

Inquiry

$ 979.00

Description

Species	Human
Expression System	HEK293
Tag	C-His-Avi
Accession Number	P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL
Synonyms	sHLA-E, MHC class I antigen E, MHC HLA-E alpha-1, MHC, HLAE, QA1, MHC HLA-E alpha-2.1
Construction	Recombinant Human HLA-E01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-Terminus. It contains Gly25-Ile305(HLA-E01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.[Accession \|P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt.-20 to -80°C for 3-6 months in unopened state after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.