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GGH/Glutamyl hydrolase gamma Protein, Human, Recombinant (His)

Catalog No. TMPY-05055
Synonyms: GGH/Glutamyl hydrolase γ, γ-glutamyl hydrolase (conjugase, folylpolyγglutamyl hydrolase), gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase), GH

gamma-Glutamyl hydrolase (GGH) regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.

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GGH/Glutamyl hydrolase gamma Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 700.00
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Biological Description
Technical Params
Product Properties
Description gamma-Glutamyl hydrolase (GGH) regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.
Species Human
Expression System HEK293
Tag His
Accession Number Q92820
Synonyms GGH/Glutamyl hydrolase γ, γ-glutamyl hydrolase (conjugase, folylpolyγglutamyl hydrolase), gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase), GH
Construction A DNA sequence encoding the human GGH (NP_003869.1) (Met1-Asp318) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity > 95 % as determined by SDS-PAGE.
Molecular Weight Approxiamtely 35.1 kDa
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Formulation Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background gamma-Glutamyl hydrolase (GGH) regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.

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Keywords

GGH/Glutamyl hydrolase gamma Protein, Human, Recombinant (His) GGH/Glutamyl hydrolase γ γ-glutamyl hydrolase (conjugase, folylpolyγglutamyl hydrolase) gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) gamma-glutamyl hydrolase conjugase, folylpolygammaglutamyl hydrolase GH recombinant recombinant-proteins proteins protein

 

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