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CD28 Protein, Rat, Recombinant (His)

(Synonyms: CD28 molecule) Copy Product Info

Synonyms: CD28 molecule

Catalog No. TMPY-04252 Copy Product Info
CD28 Protein, Rat, Recombinant (His) is expressed in HEK293 Cells. The accession number is P31042.

Product Information

Bioactivity
Bioactivity
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 μM as determined in SPR assay (Biacore T200).
Description
CD28 Protein, Rat, Recombinant (His) is expressed in HEK293 Cells. The accession number is P31042.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberP31042
ConstructionAsn20-Leu150
Protein Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsCD28 molecule
Research Background
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Chemical Properties
Molecular Weight16.66 kDa (Predicted); 40-55 kDa (Reducing conditions due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: CD28 Protein, Rat, Recombinant (His) chemical structure | CD28 Protein, Rat, Recombinant (His) molecular weight