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Synonyms: CD28 molecule

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $57 | 7-10 days | 7-10 days | |
| 10 µg | $85 | 7-10 days | 7-10 days | |
| 20 µg | $146 | 7-10 days | 7-10 days | |
| 50 µg | $285 | 7-10 days | 7-10 days | |
| 100 µg | $477 | 7-10 days | 7-10 days | |
| 200 µg | $929 | 7-10 days | 7-10 days |
| Bioactivity | Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 μM as determined in SPR assay (Biacore T200). |
| Description | CD28 Protein, Rat, Recombinant (His) is expressed in HEK293 Cells. The accession number is P31042. |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P31042 |
| Construction | Asn20-Leu150 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | CD28 molecule |
| Research Background | CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
| Molecular Weight | 16.66 kDa (Predicted); 40-55 kDa (Reducing conditions due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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