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Carbonic Anhydrase 2 Protein, Human, Recombinant (His)
(Synonyms: HEL-S-282, HEL-76, carbonic anhydrase II, Car2, CA-II, CAII, CAC) Copy Product InfoSynonyms: HEL-S-282, HEL-76, carbonic anhydrase II, Car2, CA-II, CAII, CAC
Catalog No. TMPY-01735 Copy Product Info
Purity: 98.6%
Carbonic Anhydrase 2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is P00918.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $88 | - | In Stock | |
| 10 µg | $141 | - | In Stock | |
| 20 µg | $227 | - | In Stock | |
| 50 µg | $455 | - | In Stock | |
| 100 µg | $727 | - | In Stock | |
| 200 µg | $1,170 | 7-10 days | 7-10 days | |
| 500 µg | $2,350 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:98.6%
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by its esterase activity. The specific activity is >150 pmoles/min/μg. |
| Description | Carbonic Anhydrase 2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is P00918. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-His |
| Accession Number | P00918 |
| Construction | A DNA sequence encoding the human CA2 (NP_000058.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus. Predicted N terminal: Met 1 |
| Protein Purity | ≥ 96 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.  , |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris 0.5M NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | HEL-S-282, HEL-76, carbonic anhydrase II, Car2, CA-II, CAII, CAC |
| Research Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1. |
| Molecular Weight | 30 kDa (predicted); 30 kDa (reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Carbonic Anhydrase 2 Protein, Human, Recombinant (His) chemical structure | Carbonic Anhydrase 2 Protein, Human, Recombinant (His) molecular weight
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