CAMKII beta/CAMK2B Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04408

Synonyms: calcium/calmodulin-dependent protein kinase II beta, CAM2, CAMKB, CaMKII β/CAMK2B Protein, Human, Recombinant (His & GST), calcium/calmodulin-dependent protein kinase II β, CAMK2

Calcium/calmodulin-dependent protein kinase II beta (CAMK2B) is a member of the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. CaMKII is an important player in prostate cancer cells ability to escape apoptosis under androgen ablation and facilitate the progression of prostate cancer cells to an androgen independent state. As a multifunctional protein kinase, the loss of activity may play a critical role in initiating the changes leading to ischemia-induced cell death. CaMKII are found to be important for the functions of immune cells. CaMKII can be activated by TLR ligands, and in turn promotes both myeloid differentiating factor 88 and Toll/IL-1 receptor domain-containing adaptor protein-inducing IFN-beta-dependent inflammatory responses by directly activating TAK1 and IRF3. CAMKII has four subunit isoforms (alpha, beta, gamma, delta). It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. The alpha- and beta-isoforms have narrow distributions restricted mainly to neuronal tissues, but the gamma- and delta-isoforms are ubiquitously expressed within neuronal and non-neuronal tissues. CAMK2B is important for controlling the direction of plasticity at the parallel fiber-Purkinje cell synapse. CaMK2 is involved in neuronal survival through the reorganization of the neuroarchitecture and that the regulation of this role is controlled at the level of gene expression. Because CaMK2B influences the expression of many neuroreceptors and influences neural outgrowth and pruning, its altered expression in the cerebral cortex in schizophrenia or depression may contribute to schizophrenia and depression.

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CAMKII beta/CAMK2B Protein, Human, Recombinant (His & GST)

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 498.00

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Biological Description

Technical Params

Product Properties

References and Literature

Description

Species	Human
Expression System	Baculovirus-Insect Cells
Tag	His,GST
Accession Number	Q13554-3
Synonyms	calcium/calmodulin-dependent protein kinase II beta, CAM2, CAMKB, CaMKII β/CAMK2B Protein, Human, Recombinant (His & GST), calcium/calmodulin-dependent protein kinase II β, CAMK2
Construction	A DNA sequence encoding the human CAMK2B isoform 2 (Q13554-3) (Met 1-Gln 503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 60 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 84.2 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, 0. 5mM PMSF, 10% gly, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	Calcium/calmodulin-dependent protein kinase II beta (CAMK2B) is a member of the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. CaMKII is an important player in prostate cancer cells ability to escape apoptosis under androgen ablation and facilitate the progression of prostate cancer cells to an androgen independent state. As a multifunctional protein kinase, the loss of activity may play a critical role in initiating the changes leading to ischemia-induced cell death. CaMKII are found to be important for the functions of immune cells. CaMKII can be activated by TLR ligands, and in turn promotes both myeloid differentiating factor 88 and Toll/IL-1 receptor domain-containing adaptor protein-inducing IFN-beta-dependent inflammatory responses by directly activating TAK1 and IRF3. CAMKII has four subunit isoforms (alpha, beta, gamma, delta). It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. The alpha- and beta-isoforms have narrow distributions restricted mainly to neuronal tissues, but the gamma- and delta-isoforms are ubiquitously expressed within neuronal and non-neuronal tissues. CAMK2B is important for controlling the direction of plasticity at the parallel fiber-Purkinje cell synapse. CaMK2 is involved in neuronal survival through the reorganization of the neuroarchitecture and that the regulation of this role is controlled at the level of gene expression. Because CaMK2B influences the expression of many neuroreceptors and influences neural outgrowth and pruning, its altered expression in the cerebral cortex in schizophrenia or depression may contribute to schizophrenia and depression.

References and Literature

1. Hiestand DM, et al. (1992)Calcium/calmodulin dependent protein kinase II mRNA in the gerbil brain after cerebral ischemia. Neurosci Lett. 144(1-2): 75-8. 2. Shackelford DA, et al. (1995) Effect of cerebral ischemia on calcium/calmodulin-dependent protein kinase II activity and phosphorylation. J Cereb Blood Flow Metab. 15(3): 450-61. 3. Walikonis R S, et al. (2001) Densin-180 forms a ternary complex with the (alpha)-subunit of Ca2+/calmodulin-dependent protein kinase II and (alpha)-actinin. J. Neurosci. (United States). 21 (2): 423-33. 4. Shimazaki A, et al. (2006) Calcium/calmodulin-dependent protein kinase II in human articular chondrocytes. Biorheology. 43(3-4): 223-33. 5. Novak G, et al. (2006) Increased expression of calcium/calmodulin-dependent protein kinase IIbeta in frontal cortex in schizophrenia and depression. Synapse. 59(1): 61-8. 6. Rokhlin OW, et al. (2007) Calcium/calmodulin-dependent kinase II plays an important role in prostate cancer cell survival. Cancer Biol Ther. 6(5): 732-42. 7. van Woerden GM, et al. (2009) betaCaMKII controls the direction of plasticity at parallel fiber-Purkinje cell synapses. Nat Neurosci. 2009 Jul;12(7): 823-5. .

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Keywords

CAMKII beta/CAMK2B Protein, Human, Recombinant (His & GST) calcium/calmodulin-dependent protein kinase II beta CAM2 CAM-2 CAMKB CaMKII β/CAMK2B Protein, Human, Recombinant (His & GST) calcium/calmodulin-dependent protein kinase II β CAMK-2 CAMK2 CAM 2 CAMK 2 recombinant recombinant-proteins proteins protein