AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 158 kDa. Accession number: P54619&Q9Y478&P54646
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 904.00 |
Description | AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 158 kDa. Accession number: P54619&Q9Y478&P54646 |
Species | Human |
Expression System | Baculovirus-Insect Cells |
Tag | His,GST |
Accession Number | P54619&Q9Y478&P54646 |
Construction | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 158 kDa (predicted) |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0. 5mM PMSF, 10% gly, 1mM GSH, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
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