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Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His)

Catalog No. TMPY-05311
Synonyms: E Protein

Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is AAC59274.1.

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Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His)
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 700.00
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Biological Description
Technical Params
Product Properties
Description Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is AAC59274.1.
Species DENV
Expression System Baculovirus Insect Cells
Tag C-His
Accession Number AAC59274.1
Synonyms E Protein
Construction The Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E (AAC59274.1, with mutation Thr 76 Arg, Gln 77 Glu, Trp 101 Arg, Leu 107 Arg) (Met281-Gly679) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity > 95 % as determined by SDS-PAGE.
Molecular Weight 45.7 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, pH 8.5, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

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Keywords

Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His) E Protein recombinant recombinant-proteins proteins protein

 

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