LAIR1 Protein, Rhesus macaque, Recombinant (His)

LAIR1 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-33 KDa and the accession number is A0A0U3SBU1.

Pack Size	Availability	Price/USD
10 μg	5 days	$ 86.00
50 μg	5 days	$ 262.00
500 μg	5 days	$ 2,150.00
1 mg	5 days	$ 3,220.00

Pack Size

Availability

Price/USD

Quantity

10 μg

5 days

$ 86.00

50 μg

5 days

$ 262.00

500 μg

5 days

$ 2,150.00

1 mg

5 days

$ 3,220.00

Description

LAIR1 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 25-33 KDa and the accession number is A0A0U3SBU1.

Species	Rhesus
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	A0A0U3SBU1
Synonyms	LAIR1, CD305, hLAIR1, LAIR-1, Leukocyte-Associated Immunoglobulin-Like Receptor 1
Amino Acid	Gln22-Try165
Construction	Gln22-Try165
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	25-33 KDa (reducing condition)

Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.2.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1) is a single-pass type I membrane protein. LAIR1 expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells, highly in naive T-cells and B-cells. As an inhibitory receptor, LAIR1 plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. LAIR1 plays inhibitory role independently of SH2-containing phosphatases and modulates cytokine production in CD4+ T-cells. It down-regulates IL2 and IFNG production while inducing secretion of transforming growth factor beta, also down-regulates IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. LAIR1 inhibits the differentiation of peripheral blood precursors towards dendritic cells. It also restrains proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells.

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LAIR1 Protein, Rhesus macaque, Recombinant (His)

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