Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 581.00
500 μg	5 days	$ 2,330.00
1 mg	5 days	$ 3,880.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 581.00

500 μg

5 days

$ 2,330.00

1 mg

5 days

$ 3,880.00

Description

Species	Human
Expression System	HEK293
Tag	N-His-Flag
Accession Number	P09923
Synonyms	FLJ40094, ALPI, APTNAP, EC 3.1.3.1, AP-TNAP, IAP, TNAP, HOPS, FLJ93059, MGC161443, TNSALP, Akp2
Construction	Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Val20-Asp503.[Accession \|P09923]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.