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SCR7

Name: SCR7
Brand: TargetMol
SKU: T3340
Rating: 4.5 (1 reviews)

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Catalog No. T3340Cas No. 1533426-72-0

SCR7, a specific DNA Ligase IV inhibitor, blocks nonhomologous end-joining (NHEJ).

SCR7

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Catalog No. T3340Cas No. 1533426-72-0

SCR7, a specific DNA Ligase IV inhibitor, blocks nonhomologous end-joining (NHEJ).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$185	35 days	35 days
5 mg	$520	35 days	35 days
10 mg	$896	35 days	35 days
25 mg	$1,650	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:Yellow

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Product Introduction

Bioactivity

Description	SCR7, a specific DNA Ligase IV inhibitor, blocks nonhomologous end-joining (NHEJ).
In vitro	In a Swiss albino mouse model loaded with Dalton's lymphoma, intraperitoneal injection of SCR7 (20 mg/kg) fails to reduce tumor size. Conversely, in BALB/c mice, intraperitoneal injection of SCR7 (20 mg/kg) enhances the cytotoxic effects of radiation, etoposide, and 3-aminobenzamide on derived tumors of Dalton's lymphoma cells.
In vivo	SCR7 exhibits significant inhibition of cell proliferation across various cell lines, with IC50 values reported as follows: 40 μM in MCF7 cells, 34 μM in A549 cells, 44 μM in HeLa cells, 8.5 μM in T47D cells, 120 μM in A2780 cells, 10 μM in HT1080 cells, and 50 μM in Nalm6 cells.
Kinase Assay	Complementation of SCR7 Inhibition with Puri?ed Ligase IV: Complementation experiment is carried out by adding increasing concentrations of puri?ed Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5' compatible and 5'-5' noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
Cell Research	SCR7 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3]. Wild-type, AAVS1TLR HEK293 and mouse NIH3T3 cells are maintained in DMEM supplied with 15% FBS, cells are passaged three times per week. The mouse Burkitt lymphoma cell line, generated from a Burkitt-like mouse lymphoma is maintained in DMEM supplied with 15% FBS, 2 mM HEPES, 2 mM sodium pyruvate, 2 mM L-glutamine, and 1× NAA, beta-mercaptoethanol and passaged four times per week. For puromycin selection, mCherry+ cells are sorted, seeded at 103 cells/well and selected with 3 mg/mL of Puromycin for 2 weeks. Then colonies are counted and single cells are sorted. The SCR7 inhibitor is purchased, 12 h after transfection these cells are maintained in complete medium supplied with 1 mM SCR7 inhibitor until analysis. At SCR7 concentrations of 60 mM and 10 mM, A reduction of transfection efficiency and of cell viability is observed[3].

Chemical Properties

Molecular Weight	334.4
Formula	C₁₈H₁₄N₄OS
Cas No.	1533426-72-0
Smiles	O=c1[nH]c(=S)[nH]c(\N=C\c2ccccc2)c1\N=C\c1ccccc1
Relative Density.	1.27 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (134.57 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (5.98 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9904 mL	14.9522 mL	29.9043 mL	149.5215 mL
5 mM	0.5981 mL	2.9904 mL	5.9809 mL	29.9043 mL
10 mM	0.2990 mL	1.4952 mL	2.9904 mL	14.9522 mL
20 mM	0.1495 mL	0.7476 mL	1.4952 mL	7.4761 mL
50 mM	0.0598 mL	0.2990 mL	0.5981 mL	2.9904 mL
100 mM	0.0299 mL	0.1495 mL	0.2990 mL	1.4952 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

SCR7

SCR7

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