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RG13022

Name: RG13022
Brand: TargetMol
SKU: T3545
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3545Cas No. 136831-48-6

Alias Tyrphostin RG13022, RG 13022

RG13022 (Tyrphostin RG13022) is a tyrosine kinase inhibitor; inhibits the autophosphorylation reaction of the EGF receptor (IC50: 4 μM).

RG13022

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Purity: 98.38%

Catalog No. T3545Alias Tyrphostin RG13022, RG 13022Cas No. 136831-48-6

RG13022 (Tyrphostin RG13022) is a tyrosine kinase inhibitor; inhibits the autophosphorylation reaction of the EGF receptor (IC50: 4 μM).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$33	In Stock	In Stock
10 mg	$48	In Stock	In Stock
25 mg	$113	In Stock	In Stock
50 mg	$192	In Stock	In Stock
100 mg	$303	-	In Stock
1 mL x 10 mM (in DMSO)	$35	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.38%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	RG13022 (Tyrphostin RG13022) is a tyrosine kinase inhibitor; inhibits the autophosphorylation reaction of the EGF receptor (IC50: 4 μM).
Targets&IC50	EGF:4 μM
In vitro	RG13022 suppresses EGF-stimulated cancer cell proliferation and inhibits EGF receptor autophosphorylation in immunoprecipitates (IC50: 4 μM) in a cell-free reaction. It inhibits colony formation (IC50: 1 μM) and DNA synthesis (IC50: 3 μM) in HER 14 cells stimulated by 50 ng/mL EGF, dose-dependently. Additionally, RG13022 inhibits growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. It completely blocks estrogen-stimulated phosphorylation of the EGF receptor and estrogen-induced cell proliferation, indicating that functioning TK pathways are essential for estrogen action.
In vivo	RG13022 suppresses tumor growth and extends the lifespan of tumor-bearing nude mice.
Cell Research	40 mM stock solutions of RG13022 is made in 100% DMSO and diluted with the culture medium before addition to the cells. MH-85 cells and HER 14 cells are plated in culture medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope[1].
Synonyms	Tyrphostin RG13022, RG 13022

Chemical Properties

Molecular Weight	266.29
Formula	C₁₆H₁₄N₂O₂
Cas No.	136831-48-6
Smiles	COc1ccc(\C=C(\C#N)c2cccnc2)cc1OC
Relative Density.	1.172g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (187.77 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.51 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7553 mL	18.7765 mL	37.5530 mL	187.7652 mL
5 mM	0.7511 mL	3.7553 mL	7.5106 mL	37.5530 mL
10 mM	0.3755 mL	1.8777 mL	3.7553 mL	18.7765 mL
20 mM	0.1878 mL	0.9388 mL	1.8777 mL	9.3883 mL
50 mM	0.0751 mL	0.3755 mL	0.7511 mL	3.7553 mL
100 mM	0.0376 mL	0.1878 mL	0.3755 mL	1.8777 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc