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PARP-1-IN-3

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Catalog No. T78157Cas No. 2976342-33-1

PARP-1-IN-3 is a potent PARP-1 inhibitor that inhibits PARP-1 and PARP-2 with IC50 values of 0.25 nM and 2.34 nM, respectively.PARP-1-IN-3 has potential anti-inflammatory activity, induces apoptosis, and arrests the cell cycle in the G2/M phase.PARP-1-IN-3 may be useful for the study of cancer-related diseases. cancer-related diseases.

PARP-1-IN-3

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Purity: 99.86%

Catalog No. T78157Cas No. 2976342-33-1

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$39	-	In Stock
5 mg	$62	-	In Stock
10 mg	$103	-	In Stock
25 mg	$173	-	In Stock
50 mg	$258	-	In Stock
100 mg	$380	-	In Stock
200 mg	$548	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.86%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	PARP-1-IN-3 is a potent PARP-1 inhibitor that inhibits PARP-1 and PARP-2 with IC50 values of 0.25 nM and 2.34 nM, respectively.PARP-1-IN-3 has potential anti-inflammatory activity, induces apoptosis, and arrests the cell cycle in the G2/M phase.PARP-1-IN-3 may be useful for the study of cancer-related diseases. cancer-related diseases.
Targets&IC50	SW480 cells:33.69 μM, DLD-1 cells:2.83 μM, PARP2:2.34 nM, NCM460 cells:486.87 μM, PARP1:0.25 nM, HCT116 cells:0.30 μM
In vitro	PARP-1-IN-3 (compound 13f) demonstrates effective anticancer activity with IC50 values of 0.30 μM, 2.83 μM, 33.69 μM, and 486.87 μM against HCT116, DLD-1, SW480, and NCM460 cells, respectively, following a 48-hour treatment[1]. In HCT116 cells, PARP-1-IN-3 (0.3-3 μM) inhibits colony formation and migration over 24-48 hours[1]. A 48-hour treatment with PARP-1-IN-3 (0.3-3 μM) induces the accumulation of DNA double-strand breaks in HCT116 cells[1]. Treatment with PARP-1-IN-3 (0.3-7.5 μM) for 48-72 hours results in cell cycle arrest at the G2/M phase, reduced mitochondrial membrane potential, and apoptosis in HCT116 cells[1].

Chemical Properties

Molecular Weight	425.28
Formula	C₂₁H₁₇BrN₂O₃
Cas No.	2976342-33-1
Smiles	O=C(N)C=1C=CC=CC1OCC=2C=CC=C(C2)NC(=O)C3=CC=C(Br)C=C3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (235.14 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3514 mL	11.7570 mL	23.5139 mL	117.5696 mL
5 mM	0.4703 mL	2.3514 mL	4.7028 mL	23.5139 mL
10 mM	0.2351 mL	1.1757 mL	2.3514 mL	11.7570 mL
20 mM	0.1176 mL	0.5878 mL	1.1757 mL	5.8785 mL
50 mM	0.0470 mL	0.2351 mL	0.4703 mL	2.3514 mL
100 mM	0.0235 mL	0.1176 mL	0.2351 mL	1.1757 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc