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JW74

Name: JW74
Brand: TargetMol
SKU: T15633
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15633Cas No. 863405-60-1

JW74 antagonizes LiCl-induced activation of the canonical Wnt signaling pathway (IC50: 420 nM).

JW74

😃Good

Purity: 95.00%

Catalog No. T15633Cas No. 863405-60-1

JW74 antagonizes LiCl-induced activation of the canonical Wnt signaling pathway (IC50: 420 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$34	In Stock	In Stock
10 mg	$44	In Stock	In Stock
25 mg	$98	In Stock	In Stock
50 mg	$148	In Stock	In Stock
100 mg	$208	In Stock	In Stock
200 mg	$297	-	In Stock
1 mL x 10 mM (in DMSO)	$37	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:95.00%

Appearance:solid

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	JW74 antagonizes LiCl-induced activation of the canonical Wnt signaling pathway (IC50: 420 nM).
Targets&IC50	Wnt:420 nM
In vitro	Ki-67 expression is reduced from 97.5% in DMSO-treated cells to 86.7% in JW74-treated cells. Relative to DMSO-treated cells, the cellular viability of U2OS cells treated for 72 h treatment with 10 μM JW74 is reduced to 80%. The effect of tankyrase inhibition on cellular viability is tested by performing an MTS assay. Flow cytometry is also performed to determine the expression marker Ki-67 in U2OS following 48 h treatment with DMSO or 10 uM JW74 [2]. JW74 displays a reduction of canonical Wnt signaling in the ST-Luc assay (IC50: 790 nM) [1].
In vivo	The in vivo efficacy of JW74 is evaluated using SW480 cell xenografts, with high doses (150 or 300 mg/kg) administered due to rapid degradation in the organism, as shown by human liver microsome analysis (t1/2=2.5 minutes) and pharmacokinetic analyses (t1/2=30 minutes after peroral injections and t1/2=15 minutes after intravenous injections). JW74 concentrations in tumors range from 4.2 to 72.1 μmol/kg for 150 mg/kg, 1.9 to 11.1 μmol/kg for 300 mg/kg, and reach 2.8 μM in plasma for both doses [1].

Chemical Properties

Molecular Weight	456.52
Formula	C₂₄H₂₀N₆O₂S
Cas No.	863405-60-1
Smiles	COc1ccc(cc1)-n1c(SCc2nc(no2)-c2ccc(C)cc2)nnc1-c1ccncc1
Relative Density.	1.35 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (98.57 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.38 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1905 mL	10.9524 mL	21.9048 mL	109.5242 mL
5 mM	0.4381 mL	2.1905 mL	4.3810 mL	21.9048 mL
10 mM	0.2190 mL	1.0952 mL	2.1905 mL	10.9524 mL
20 mM	0.1095 mL	0.5476 mL	1.0952 mL	5.4762 mL
50 mM	0.0438 mL	0.2190 mL	0.4381 mL	2.1905 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc