Shopping Cart

Your shopping cart is currently empty

Epibrassinolide

Name: Epibrassinolide
Brand: TargetMol
SKU: T5514
Price: 54 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

🥰Excellent

Catalog No. T5514Cas No. 78821-43-9

Alias BP55, B1105, 24-Epibrassinolide

Epibrassinolide (BP55) (EBR) is a biologically active compound of the brassinosteroids, is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids.

Epibrassinolide

Copy Product Info

🥰Excellent

Purity: 98.11%

Catalog No. T5514Alias BP55, B1105, 24-EpibrassinolideCas No. 78821-43-9

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$54	In Stock	In Stock
10 mg	$76	In Stock	In Stock
25 mg	$119	In Stock	In Stock
50 mg	$163	In Stock	In Stock
100 mg	$228	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$58	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:98.11%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Epibrassinolide (BP55) (EBR) is a biologically active compound of the brassinosteroids, is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids.
In vitro	Epibrassinolide(EBR) was shown as a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth[1].It has been shown to protect neuronal PC12 cells from 1-methyl-4-phenylpyridinium-induced oxidative stress and consequent apoptosis in dopaminergic neurons[2]
Cell Research	Cells were seeded at 1*10^4 density per well in 96-well plates and exposed to various concentrations of EBR for 24 and 48 h. 10 ul 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (5 mg/ml) ?was added to each well and cells were kept at 37 C for 4 h. The resulting formazan crystals were solubilized in 200 ul DMSO.?The density of the solubilized formazan was read at 570 nm spectrophotometrically.
Synonyms	BP55, B1105, 24-Epibrassinolide

Chemical Properties

Molecular Weight	480.68
Formula	C₂₈H₄₈O₆
Cas No.	78821-43-9
Smiles	CC(C)[C@@H](C)[C@@H](O)[C@H](O)[C@@H](C)[C@H]1CC[C@H]2[C@@H]3COC(=O)[C@H]4C[C@H](O)[C@H](O)C[C@]4(C)[C@H]3CC[C@]12C
Relative Density.	1.141 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 48.1 mg/mL (100.07 mM), Sonication and heating are recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0804 mL	10.4019 mL	20.8039 mL	104.0193 mL
5 mM	0.4161 mL	2.0804 mL	4.1608 mL	20.8039 mL
10 mM	0.2080 mL	1.0402 mL	2.0804 mL	10.4019 mL
20 mM	0.1040 mL	0.5201 mL	1.0402 mL	5.2010 mL
50 mM	0.0416 mL	0.2080 mL	0.4161 mL	2.0804 mL
100 mM	0.0208 mL	0.1040 mL	0.2080 mL	1.0402 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc