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BT2

Name: BT2
Brand: TargetMol
SKU: T14834
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14834 Copy Product Info

Purity: 99.86%

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BT2, a BCKDC kinase (BDK) inhibitor, exhibits an IC50 of 3.19 μM and functions as a potent, selective Mcl-1 inhibitor with a Ki value of 59 μM. Its interaction with BDK induces helix movements in the N-terminal domain, leading to BDK's dissociation from the branched-chain α-ketoacid dehydrogenase complex (BCKDC).

Cas No. 34576-94-8

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Pack Size	Price	USA Stock	Global Stock
10 mg	$39	In Stock	In Stock
25 mg	$58	In Stock	In Stock
50 mg	$72	In Stock	In Stock
100 mg	$105	In Stock	In Stock
500 mg	$256	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.86%

Appearance:Solid

Color:White to Yellow

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	BT2, a BCKDC kinase (BDK) inhibitor, exhibits an IC50 of 3.19 μM and functions as a potent, selective Mcl-1 inhibitor with a Ki value of 59 μM. Its interaction with BDK induces helix movements in the N-terminal domain, leading to BDK's dissociation from the branched-chain α-ketoacid dehydrogenase complex (BCKDC).
Targets & IC50	BDK:3.19 μM (IC50), MCL1:59 μM (Ki)
In vivo	BT2 treatment reduces the protein levels of BDK in kidneys and heart. The -fold activation of BCKDC activity in the above tissues correlates with decreased phosphorylation in heart, muscle, and kidney after the long term BT2 treatment. BT2 (20 mg/kg/day; i.p.; daily; for 7 days; C57BL/6J male mice) treatment robustly enhances BCKDC activity in the heart (12.3-fold) compared with the vehicle-treated animals. Less activation is obtained in muscle and kidney at 3.6- and 3.8-fold, respectively [1].

Chemical Properties

Molecular Weight	247.1
Formula	C₉H₄Cl₂O₂S
Cas No.	34576-94-8
Smiles	OC(=O)c1sc2cc(Cl)ccc2c1Cl
Relative Density.	1.653 g/cm3

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 82.5 mg/mL (333.87 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: < 8.33 mg/mL (33.71 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% Corn oil: 8.33 mg/mL (33.71 mM), Solution.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: < 8.33 mg/mL (33.71 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% (20% SBE-β-CD in Saline): < 8.33 mg/mL (33.71 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0469 mL	20.2347 mL	40.4694 mL	202.3472 mL
5 mM	0.8094 mL	4.0469 mL	8.0939 mL	40.4694 mL
10 mM	0.4047 mL	2.0235 mL	4.0469 mL	20.2347 mL
20 mM	0.2023 mL	1.0117 mL	2.0235 mL	10.1174 mL
50 mM	0.0809 mL	0.4047 mL	0.8094 mL	4.0469 mL
100 mM	0.0405 mL	0.2023 mL	0.4047 mL	2.0235 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc