Shopping Cart

Your shopping cart is currently empty

AMG-548

Name: AMG-548
Brand: TargetMol
SKU: T10298L
Price: 55 USD
Availability: InStock
Rating: 5 (10 reviews)

😃Good

Catalog No. T10298LCas No. 864249-60-5

Alias AMG548

AMG-548 is a selective and orally active p38α MAPK inhibitor (Ki = 0.5 nM), with higher selectivity than p38β, p38γ, and p38δ, and can also inhibit the TNFα, CK1δ/ε, and Wnt signalling pathways.

AMG-548

😃Good

Purity: 99.91%

Catalog No. T10298LAlias AMG548Cas No. 864249-60-5

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$55	-	In Stock
5 mg	$125	-	In Stock
10 mg	$182	-	In Stock
25 mg	$430	-	In Stock
100 mg	$1,660	-	In Stock
200 mg	$2,230	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.91%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	AMG-548 is a selective and orally active p38α MAPK inhibitor (Ki = 0.5 nM), with higher selectivity than p38β, p38γ, and p38δ, and can also inhibit the TNFα, CK1δ/ε, and Wnt signalling pathways.
Targets&IC50	p38α:0.5 nM (ki), JNK1:11480 nM (ki), JNK2:39 nM (ki), p38α (dog):5.0 nM (ki), p38β:3.6 nM (ki), JNK3:61 nM (ki), p38γ:4100 nM (ki), p38δ:2600 nM (ki)
In vitro	Methods: U2OS-EFC cells were treated with AMG-548 (0.01-0.1 μM) to investigate its ability to mediate Wnt-3a-induced hDvl2 mobility shift. Results: AMG-548 inhibited hDvl2 displacement at micromolar concentrations. [2]
In vivo	Experimental data showed that AMG-548 exhibited differentiated pharmacokinetic characteristics in different animal models: the elimination half-life (t1/2) in rats was 4.6 hours, while in canine models it was extended to 7.3 hours. In terms of bioavailability (F value), the compound reached 62% in rats, while it was relatively low at 47% in dogs. These Results indicate that AMG-548 has significant species differences, and its metabolic clearance rate and absorption efficiency vary significantly between different animal models. [1]
Synonyms	AMG548

Chemical Properties

Molecular Weight	461.56
Formula	C₂₉H₂₇N₅O
Cas No.	864249-60-5
Smiles	O=C1C(=C(N=C(NC[C@H](CC2=CC=CC=C2)N)N1C)C=3C=CN=CC3)C4=CC5=C(C=C4)C=CC=C5
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (173.33 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1666 mL	10.8328 mL	21.6657 mL	108.3283 mL
5 mM	0.4333 mL	2.1666 mL	4.3331 mL	21.6657 mL
10 mM	0.2167 mL	1.0833 mL	2.1666 mL	10.8328 mL
20 mM	0.1083 mL	0.5416 mL	1.0833 mL	5.4164 mL
50 mM	0.0433 mL	0.2167 mL	0.4333 mL	2.1666 mL
100 mM	0.0217 mL	0.1083 mL	0.2167 mL	1.0833 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc