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USP7/USP47 inhibitor

Catalog No. T4338 CAS 1247825-37-1

Synonyms: USP7/47 inhibitor-1

USP7/USP47 inhibitor (USP7/47 inhibitor-1) is a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, with EC50s of 0.42 μM and 1.0 μM, respectively.

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USP7/USP47 inhibitor, CAS 1247825-37-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 106.00
2 mg	In stock	$ 190.00
5 mg	In stock	$ 347.00
10 mg	In stock	$ 497.00
25 mg	In stock	$ 793.00
50 mg	In stock	$ 1,130.00
100 mg	In stock	$ 1,520.00
500 mg	In stock	$ 2,970.00
1 mL * 10 mM (in DMSO)	In stock	$ 360.00

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Purity: 98.5%

Purity: 97.98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	USP7/USP47 inhibitor (USP7/47 inhibitor-1) is a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, with EC50s of 0.42 μM and 1.0 μM, respectively.
Targets&IC50	USP47:1.0 μM(EC50), USP7:0.42 Μm(EC50)
In vitro	USP7/USP47 inhibitor does not inhibit caspase 3, calpain 1, 20S proteasome, and a panel of representative USPs (USP2, USP5, USP8, USP21, and USP28; EC50 > 31.6 μM). USP7/USP47 inhibitor inhibits the growth of HCT-116 cells with an EC50 of 7.6 μM.
Kinase Assay	The cloning, expression and purification of USP21 from BL21 (DE3) bacteria are performed using standard molecular biology techniques. USP2, USP5, USP7, USP8, USP28, USP47, Ub-PLA2 (Ub-CHOP) and Ub-EKL (Ub-CHOP2) are generated. Caspase 3 and the caspase 3 substrate DEVD-Rh110 are used. Deubiquitylating enzyme, cathepsin B and 20S proteasome chymotrypsin like protease activities are measured. Caspase 3 activity is determined using a similar protocol. Briefly, dose ranges of compound (including USP7/USP47 inhibitor) are incubated with caspase 3 for 30 minutes before the addition of DEVD-Rh110 and reading on a fluorometric plate reader using excitation and emission maxima of 485 nm and 531 nm respectively. The final concentrations of caspase 3 and DEVD-Rh110 are 2 nM and 100 nM respectively

Synonyms	USP7/47 inhibitor-1
Molecular Weight	484.4
Formula	C18H11Cl2N3O3S3
CAS No.	1247825-37-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 44 mg/mL

References and Literature

1. Weinstock J, et al. Selective Dual Inhibitors of the Cancer-Related Deubiquitylating Proteases USP7 and USP47. ACS Med Chem Lett. 2012 Sep 11;3(10):789-92.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library HIF-1 Signaling Pathway Compound Library Bioactive Compounds Library Max Cell Cycle Compound Library Target-Focused Phenotypic Screening Library Ubiquitination Compound Library Bioactive Compound Library NO PAINS Compound Library Anti-Cancer Compound Library DNA Damage & Repair Compound Library

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GRL0617 USP7-IN-3 6-Thioguanine IU1 USP7-IN-6 LDN-91946 Spautin-1 C527

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Keywords

USP7/USP47 inhibitor 1247825-37-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB USP-7/USP47 inhibitor Deubiquitinase inhibit Inhibitor USP7/47 inhibitor-1 DUBs inhibitor

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